茄子单性结实近等基因系的创建及遗传背景分析
DOI:
作者:
作者单位:

1.重庆市农业科学院;2.重庆市农村土地整治中心

作者简介:

通讯作者:

中图分类号:

基金项目:

重庆市自然科学基金面上项目(cstc2019jcyj-msxmX0541);现代农业产业技术体系专项资金资助(CARS-23-A12);重庆市农业科学院绩效激励引导专项(cqaas2019jxjl01)


Construction and genetic background analysis of eggplant near-isogenic lines
Author:
Affiliation:

1.Vegetable Research Institute,Chongqing Academy of Agricultural Sciences;2.Chongqing Rural Land Improvement Center

Fund Project:

Supported by the Natural Science Foundation of Chongqing (cstc2019jcyj-msxmX0541); earmarked fund for China Agriculture Research System (CARS-23-A08); earmarked fund for performance incentive and guidance of Chongqing Academy of Agricultural Sciences (cqaas2019jxjl01)

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    为了探索单性结实分子调控网络,准确获得控制单性结实性状的基因,为茄子单性结实育种提供基础材料,构建茄子单性结实/非单性结实性近等基因系。试验采用杂交、回交和自交技术,结合单性结实鉴定和农艺性状调查,获得两组近等基因系JDX1和JDX8。利用覆盖茄子基因组的219对SSR引物对茄子近等基因系及亲本进行遗传背景分析。结果表明,近等基因系JDX1和JDX8与轮回亲本的遗传背景相似度分别达97.14%和88.23%,说明两组近等基因系材料遗传背景与其轮回亲本基本一致。单性结实鉴定结果表明,与轮回亲本比,近等基因系JDX1与JDX8已经完全转育成非单性结实株系。对两组单性结实近等基因系农艺性状调查结果表明,轮回亲本相比,JDX1和JDX8株高、首花高度、节间距、叶片长和宽、果实长和粗均不存在显著差异。结合果色、株型等农艺性状,可以得出单性结实近等基因系JDX1和JDX8的表型基本与轮回亲本一致的结论。该材料的成功创制为单性结实基础研究奠定良好的材料基础。

    Abstract:

    To explore the mechanism of regulation network of Parthenocarpy, accurately obtain the genes controlling parthenocarpy traits, provide basic materials for parthenocarpy breeding of eggplant, the parthenocarpy/non parthenocarpy near isogenic line of eggplant was constructed. Two groups of near isogenic lines (NILs) JDX1 and JDX8 were obtained by hybridization, backcross and selfing techniques, combined with parthenocarpy identification and agronomic traits investigation. 219 pairs of SSR primers covering eggplant genome were used to analyze the genetic background of eggplant NILs and parents. The results showed that the genetic background similarity between proximal JDX1 and JDX8 and reincarnation parents was 97.14% and 88.23%, respectively, indicating that the genetic background of the two NILs materials was basically consistent with their reincarnation parents. The Parthenocarpy identification showed that compared with recurrent parents, JDX1 and JDX8 had been completely transformed into non parthenocarpic lines. The results showed that there were no significant differences in plant height, first flower height, node spacing, leaf length and width, fruit length and thickness between NILs and their recurrent parents.Combined with the agronomic characteristics such as fruit color and strain type, it can be concluded that the JDX1 and JDX8 is basically consistent with the reincarnation parent. The successful creation of this material lays a good material foundation for the basic research of single solidity.

    参考文献
    相似文献
    引证文献
引用本文
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2022-03-09
  • 最后修改日期:2022-03-24
  • 录用日期:2022-04-07
  • 在线发布日期:
  • 出版日期:
您是第位访问者
京ICP备09069690号-5
植物遗传资源学报 ® 2022 版权所有
技术支持:北京勤云科技发展有限公司