Foot rot of sweetpotato caused by Diaporthe destruens is a new disease in the southeast coast of Zhejiang Province. In order to identify resistant germplasm resources and explore the method of resistance identification in natural induced field, 126 sweetpotato germplasm resources were identified, the changes of disease symptom in late growth period of sweetpotato and effects of resistance on yield loss rate of fresh storage root were analyzed. The results showed that the control variety Zheshu255 was high resistant, Zheshu 38 was resistant and Zheshu 13 was high susceptible. Among the 89 varieties, there was no high resistance germplasm, only 3 germplasms were resistant, and over 65% of germplasms were high susceptible. 13 resistant germplasms were identified from 34 hybrid progenies of resistant variety Zheshu255, including 6 high resistant germplasms and 2 resistant germplasms. There were significant changes of disease symptom in the late growth period of sweet potato, especially the disease index of moderate resistant and susceptible germplasm sharply increased from the growth period of 120 d to 135 d. The disease grade of 93.8% of the total susceptible plants was 4 at the growth period of 135 d. Most of the susceptible plants finally rotted or died at the stem. The yield loss rate of sweet potato with good resistance (high resistance or resistance) could be controlled below 15% , while the yield loss rate of susceptible varieties was over 65% , even no harvest. In a word, the resistant germplasm resources of foot rot disease of sweetpotato were scarce, the good resistant breeding materials could be obtained through the routine hybridization of the resistant parents, and the incidence and disease index might be used as the identification index of resistance to foot rot of sweetpotato in natural induced field.