大白菜和结球甘蓝有益硫苷及MAM基因表达分析

大白菜和结球甘蓝有益硫苷及MAM基因表达分析
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作者单位:1.华北作物改良与调控国家重点实验室/河北省蔬菜种质创新与利用重点实验室/河北省产业协同创新中心/河北农业大学园艺学院;2.北京市农林科学院蔬菜研究中心／农业农村部华北地区园艺作物生物学与种质创制重点实验室;3.中国农业科学院蔬菜花卉研究所
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基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目）

The correlation analysis of beneficial glucosinolate and MAM gene expression in Brassica rapa ssp. pekinensis and B. oleracea var.capitata

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1.State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei/Collaborative Innovation Center of Vegetable Industry in Hebei/ College of Horticulture,Hebei Agricultural University;2.China;3.Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops North China,Ministry of Agriculture;4.Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences

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The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)

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硫苷及其降解产物是十字花科蔬菜重要的次生代谢产物,具有防癌抗癌作用。硫苷合成基因MAMs(Methylthioalkylmalate synthases)负责硫苷侧链的延伸,MAM1催化生成短链脂肪族硫苷而MAM3催化生成长链脂肪族硫苷,因此MAM基因影响了硫苷成分的多样性。本研究利用大白菜和结球甘蓝自交系,从苗期到莲座期取样测定硫苷含量及MAM的相对表达量,分析有益硫苷在两者之间的积累差异以及MAM基因的表达调控作用。结果表明：从苗期到莲座期结球甘蓝的硫苷含量均高于大白菜,大白菜中侧链长度为5C和4C脂肪族硫苷(GBN和NAP)含量较高,而结球甘蓝中3C和4C脂肪族硫苷(IBE、SIN和GRA)含量较高,有益硫苷SIN和GRA在结球甘蓝中的含量显著高于大白菜。在大白菜和结球甘蓝中分别发现7个MAM拷贝基因,同源基因比较发现MAM1和MAM3的相对表达量在两者之间存在显著差异。大白菜中BraMAM1.1和BraMAM1.2的表达量显著低于结球甘蓝同源基因BoMAM1.1和BoMAM1.2的表达量,而BraMAM3.2和BraMAM3.3的表达量显著高于结球甘蓝同源基因BoMAM3.1的表达量；并且结球甘蓝中3C脂肪族硫苷含量与BoMAM1.1的表达量呈显著正相关,大白菜中5C脂肪族硫苷含量与BraMAM3.2的表达量呈显著正相关。由此推测可能是BraMAM1.1的低表达而BraMAM3.2的高表达,导致了大白菜中3C脂肪族硫苷SIN积累极少而5C脂肪族硫苷GBN积累较多。

Abstract:

Glucosinolates and their degradation products are important secondary metabolite of cruciferous vegetables with the function of anti-cancer. MAMs (Methylthioalkylmalate synthases) are involved in glucosinolate side-chain elongation. MAM1 catalyzes the aliphatic glucosinolate synthesis with short-chain, while MAM3 catalyzes that with long-chain. In this study, the glucosinolate content and the relative expression of MAM genes was analyzed in Chinese cabbage and cabbage inbred lines during seedling and rosette stages to analyze the relationship between MAM gene expression and the accumulation of beneficial glucosinolates. The results showed that the glucosinolate content in cabbage was higher than that in Chinese cabbage from seedling to rosette stage. The content of 5C and 4C aliphatic glucosinolates (GBN and NAP) were dominant in Chinese cabbage, whereas the main glucosinolates in cabbage were 3C and 4C aliphatic glucosinolates (IBE, SIN and GRA). The anti-cancer component SIN and GRA was significantly higher in cabbage than that in Chinese cabbage. The genome of Chinese cabbage and cabbage contains seven MAM paralogues each. By analyzing the expression levels of MAMs, we found that there was a significant difference in the relative expression level of MAM1 and MAM3 between Chinese cabbage and cabbage. The expression level of BraMAM1.1 and BraMAM1.2 in Chinese cabbage was significantly lower than that of BoMAM1.1 and BoMAM1.2 in cabbage, while the expression level of BraMAM3.2 and BraMAM3.3 was significantly higher than that of BoMAM3.1. Moreover, the content of 3C aliphatic glucosinolates in cabbage was positively correlated with the expression of BoMAM1.1, and the content of 5C aliphatic glucosinolates in Chinese cabbage was positively correlated with the expression of BraMAM3.2. We speculated that the low expression of BraMAM1.1 and the high expression of BraMAM3.2 may lead to the low accumulation of 3C aliphatic glucosinolates (SIN) and the high accumulation of 5C aliphatic glucosinolates (GBN) in Chinese cabbage.

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收稿日期:2022-02-19
最后修改日期:2022-03-26
录用日期:2022-03-28
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