利用一个F2:3单基因分离群体精细定位水稻穗顶部退化基因ATS1
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1.六安职业技术学院;2.安徽农业大学农学院;3.黑龙江省农业科学院耕作栽培研究所;4.中国农业科学院作物科学研究所

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国家自然科学基金项目(面上项目)


Fine mapping of rice panicle apical abortion gene ATS1 using one F2:3 single-gene-segregating population
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Affiliation:

1.School of City Comstruction,Lu'2.'3.an Vocational College,Lu’an;4.College of Agronomy,Anhui Agricultural University;5.Institute of Crop cultivation and Tillage,Heilongjiang Academy of Agricultural Sciences;6.The Chinese academy of agricultural sciences institute of crop science

Fund Project:

The National Natural Science Foundation of China (General Program)

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    摘要:

    水稻穗顶部退化是一种典型的数量性状,由基因型和生长环境共同决定,极容易受环境条件的影响。Ats1是一个来自于秋光和七山占的染色体片段置换系,先前的连锁分析发现,它在第8染色体上带有一个穗顶部退化基因ATS1,和我们之前定位的qPAA8位置近似。由于精细定位的结果难以通过转基因验证,我们猜测取样单株表型与基因型不一致导致了精细定位结果不准确。通过比较北京地区2018年与其它年份的气候差异,以及在不同生长环境条件下Ats1的穗顶部退化表型时发现,穗顶部退化表型与生长环境条件高度关联。在2018年的高温生长条件下,Ats1穗顶部退化的严重程度与普通年份相比明显减轻,说明高温降低了Ats1穗顶部退化表型的发生率。另外,通过对新创建杂交组合IRAT129′Ats1的F2群体的遗传分析时发现,F2群体中穗顶部退化的遗传分离明显偏离单基因显性遗传的3退化:1正常的分离比例,说明Ats1置换系中还存在其它穗顶部退化基因。在本文中,我们基于F2:3家系群的表型分析,选出了单基因分离家系18C4013,并在此基础上,进一步将ATS1基因范围缩小至包含4个潜在候选基因的57Kb区域,为最终克隆该基因奠定了基础,该方法可以被进一步用于其它受环境条件影响的复杂性状的基因精细定位。

    Abstract:

    The panicle apical abortion (PAAB) of rice is a typical quantitative trait that is determined by both genotypes and growth environments, and their interactions, particularly, influenced by the ambient temperature. A chromosome segment substitution line Ats1 (aborted top spikelet mutant 1) originated from the cross-combination of Qiuguang ′ Qishanzhan has been used for map-based cloning of PAAB gene ATS1. Previous linkage analysis suggested that the candidate gene of AST1 was located on chromosome 8, sharing a long fragment of positioning interval with that of unidentified gene qPAA8. Due to the failure at the corroboration of candidate genes by transgenic test, we speculated that the results of fine mapping is inaccurate ascribe to the deviation of phenotype with the genotype of the sampled individuals. By comparing the climate differences between 2018 and other years in Beijing and the phenotype of Ats1 under different growth environment conditions, we found that the PAAB severity of Ats1 growth under the high temperature in 2018 was significantly alleviated compared with ordinary years, indicating that high temperature reduced the incidence of PAAB in Ats1. In addition, through the genetic analysis to the F2 population of newly created cross-combination IRAT129′ats1, we further found that the genetic separation of PAAB obviously deviated from ratio of the single gene dominant inheritance, i.e. 3 PAAB to 1 normal, indicating that there is extra PAAB genes involving. Here we provided a convenient strategy on developing a single gene segregating population (SGSP), based on the phenotypic analysis in combination with marker-associated selection to individuals of F2:3 lines. Finally, we finally narrowed the candidate gene of ATS1 into a 57 Kb region including four potential candidate genes, settling a foundation for the final cloning of the target gene. This method could be helpful using in fine mapping on other complex traits easily affected by environmental conditions.

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  • 收稿日期:2022-01-12
  • 最后修改日期:2022-02-21
  • 录用日期:2022-03-09
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