苦荞转录因子FtDREB6基因克隆及其抗旱功能鉴定
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1.河北农业大学园林与旅游学院;2.中国农业科学院作物科学研究所

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河北省教育厅青年拔尖人才项目(BJ2019022);保定市科技支撑计划项目(2011ZN001)


Cloning of Transcription Factor FtDREB6 in Tartary Buckwheat (Fagopyrum tataricum) and Identification of its Function in Drought Resistance
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College of Landscape Architecture and Tourism,Agricultural University of Hebei

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The Top Young talents Project of the Department of Education of Hebei Province(BJ2019022);Baoding Science and Technology support Plan Project(2011ZN001)

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    摘要:

    AP2/ERF转录因子家族在植物逆境胁迫中发挥重要作用,本文以‘品苦1号’为材料,从中克隆得到FtDREB6基因,该基因CDS全长615 bp,编码204个氨基酸,编码蛋白分子量22.7 KDa,等点位点为4.96。在Tair网站上将其与拟南芥进行同源比对,发现其与拟南芥AtERF043同源性较高。转录激活活性分析表明FtDREB6全长没有自激活活性。利用农杆菌介导法将FtDREB6基因转入拟南芥,过表达FtDREB6基因显著提高转基因拟南芥的抗旱性。将FtDREB6基因转入发根农杆菌A4,侵染苦荞外植体诱导毛状根,在甘露醇处理下,过表达毛状根的SOD和CAT活性显著高于对照组A4且MDA含量显著低于对照组。以上结果表明,FtDREB6参与苦荞干旱胁迫反应,为进一步研究苦荞抗旱分子机制提供了参考。

    Abstract:

    AP2/ERF transcription factor family have been identified to play important roles in abiotic stress responses. In this study, the full-length coding sequence of FtDREB6 gene was cloned with a length of 615 bp, encoding for 204 amino acids residues, a molecular weight of 22.7 kDa and an isoelectric point of 4.96 from tartary buckwheat (Fagopyrum tataricum) cultivar “Pinku 1”. The sequence of FtDREB6 had higher homology with Arabidopsis thaliana AtERF043 by the sequence alignment on Tair website. FtDREB6 gene was showed to lost transcription activity by transactivation analysis. Moreover, the coding sequence of FtDREB6 was transformed into Arabidopsis thaliana by Agrobacterium mediated transformation, the drought resistance of Arabidopsis was significantly increase by the overexpression of FtDREB6. The expression vector containing FtDREB6 gene was transformed into Agrobacterium rhizogenes A4, which infects explant for inducing the hairy roots. The superoxide dismutase activity and catalase activity in overexpressing hairy roots were significantly higher than the control, and the content of malondialdehyde significantly lower than the control under D-Mannitol treatment. These results indicated that FtDREB6 was involved in responses to drought stress, which providing a reference for further study on molecular mechanism for drought tolerance in tartary buckwheat.

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  • 收稿日期:2021-12-21
  • 最后修改日期:2022-03-02
  • 录用日期:2022-03-09
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