甘蓝型油菜HIPPs基因家族的鉴定与表达分析
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1.贵州师范大学生命科学学院;2.中国农业科学院油料作物研究所/农业部油料作物生物学与遗传育种重点实验室

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基金项目:

国家自然科学基金(31801391,32060463);贵州省科技厅基金([2020]1Y102);贵州省科技厅基础研究项目([2019]1223)


Identification and Expression Analysis of HIPPs Gene Family in Brassica napus L.
Author:
Affiliation:

1.School of Life Sciences, Guizhou Normal University;2.Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture

Fund Project:

National Natural Science Foundation of China (31801391,32060463),Fund of Guizhou Science and Technology Departm ([2020]1Y102),Natural Science Foundation of Guizhou Province (Qiankehejichu[2019]1223)

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    摘要:

    The heavy metal-associated isoprenylated plant proteins (HIPPs), which play an important role in the maintenance of metal ion homeostasis and detoxification mechanism, have been accepted as key proteins in the safety transportation of metallic ions in plants. While the HIPPs gene family has been investigated in these model plants, such as Oryza sativa L. and Arabidopsis thaliana L., to our knowledge the HIPPs gene family in Brassica napus L remains analyzed. In this study, 104 HIPPs genes were identified in the genome of Brassica napus L. (Darmor-bzh v10) using Bioinformatics methods. The HIPPs members were not evenly distributed on 19 chromosomes. The amino acid number, protein molecular weight, isoelectric point, and protein hydrophobicity index of BnaHIPPs were ranged from 120 to 630 aa, 13 827.56 to 64 467.05 Da, 8.07, and -1.146 to -0.17, respectively. The HIPPs genes showed tissue specific expression and temporal specificity, as well as the highest transcripts in roots. Moreover, the expression of BnaHIPPs was significantly altered in response to Cd2+ stress treatment. For example, BnaA08p24150 and BnaA09p41730 were up-regulated in both roots and leaves. The phylogenetic analysis showed that BnaHIPPs were divided into five distinct clades. The collinearity analysis elucidated that the expansion of the BnaHIPPs gene family members was largely contributed by fragment replication, and only for four members BnaC05p3800, BnaC05p3810, BnaC05p3820, and BnaC05p3830 were found with tandem repetition. Our results will provided insights for future deciphering the BnaHIPPs gene family members in B. napus L..

    Abstract:

    HIPPs (heavy metal-associated isoprenylated plant proteins) are key proteins for the safety transportation of metallic ions in the plants cell, which plays an important role in the maintenance of metal ion homeostasis and detoxification mechanism. To date, most studies on HIPPs gene family focus on model plants Oryza sativa L. and Arabidopsis thaliana L., while relatively few reports have been reported in Brassica napus L.. In this study, In this study, 104 HIPPs genes were identified in the genome of Brassica napus L. (Darmor-bzh v10) using Bioinformatics methods, which were unevenly distributed on 19 chromosomes of B. napus L.. By analyzing the physicochemical properties, we found that the amino acid number, protein molecular weight and protein hydrophobicity index of BnaHIPPs genes ranged from 120 to 630 aa, 13 827.56 to 64 467.05 Da, and -1.146 to -0.17, respectively. And the average isoelectric point was 8.07. Their expression patterns have certain spatiotemporal expression properties in different tissues and developmental periods, with the highest expression in roots. The expression pattern of BnaHIPPs were altered in response to Cd2+ stress treatment, and the relative expression levels of BnaA08p24150 and BnaA09p41730 were up-regulated in both roots and leaves. The phylogenetic relationship confirmed that 104 BnaHIPPs gene were divided into five distinct clades. In addition, collinearity analysis elucidated that during the evolution of BnaHIPPs gene famliy, most BnaHIPPs participate in the expansion of the BnaHIPPs gene family in the form of fragment replication, and only BnaC05p3800, BnaC05p3810, BnaC05p3820 and BnaC05p3830 were found to involve in the replication events of BnaHIPPs in the form of tandem repetition. Our results will lay the foundation for further research on the BnaHIPPs gene family in B. napus L..

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钱 方,胡利娟,余 婕,等.甘蓝型油菜HIPPs基因家族的鉴定与表达分析[J].植物遗传资源学报,2022,23(4):1187-1201.

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  • 收稿日期:2021-11-30
  • 最后修改日期:2022-01-24
  • 录用日期:2022-02-09
  • 在线发布日期: 2022-07-08
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