大麦HvMBF1c克隆及其响应盐胁迫的表达模式研究
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1.省部共建干旱生境作物学国家重点实验室/甘肃省作物遗传改良与种质创新重点实验室;2.河南农业大学林学院;3.甘肃农业大学农学院

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财政部和农业农村部:国家现代农业产业技术体系项目(CARS-05-04B-2);甘肃省教育厅产业支撑计划项目(2021CYZC-12);国家自然科学基金项目(31860377);甘肃农业大学伏羲青年英才计划(Ganfx-03Y06);甘肃省青年人才托举工程;甘肃省科技支撑计划项目(1604NKCA052-2);甘肃省科技重大专项(17ZD2NA016)


Cloning of Barley HvMBF1c and Analysis of Its Expression Pattern in Response to Salt Stress Treatment
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Affiliation:

1.State Key Laboratory of Aridland Crop Science (Gansu Agricultural University)/Gansu Provincial Key Laboratory of Crop Improvement & Germplasm Enhancement;2.College of Forestry, Henan Agricultural University;3.College of Agronomy, Gansu Agricultural University

Fund Project:

China Agriculture Research System of MOF and MARA (CARS-05-04B-2), Industrial Support Project of Gansu Provincial Education Department (2021CYZC-12), National Natural Science Foundation (31860377), Fuxi Young Talents Program of Gansu Agricultural University (Ganfx-03Y06), Gansu Province Youth Talent Promotion Project, Gansu Science and Technology Support Program (1604NKCA052-2), Important Special Project for Science and Technology of Gansu Province (17ZD2NA016)

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    摘要:

    为探究大麦耐盐相关基因HvMBF1c的潜在功能及响应盐胁迫的表达规律,本研究以大麦种质ZY26(耐盐型)和ZY218(盐敏感型)为试验材料,采用同源克隆法克隆HvMBF1c基因,运用生物信息学软件预测分析该基因及其编码蛋白的结构、理化性质、同源蛋白差异及系统进化。结果表明,HvMBF1c包含471bp的开放阅读框,编码156个氨基酸,位于大麦染色体7HL上,无内含子结构;其编码蛋白主要由N端MBF1结构域和C端HTH结构域组成,具有不稳定、碱性、亲水性、非跨膜、无信号肽结构等特性,二级结构以α螺旋为主;进化分析表明HvMBF1c与多种植物MBF1的氨基酸序列高度同源,与小麦TaMBF1c的亲缘关系最近;亚细胞定位表明该基因主要定位于细胞核和细胞质中。qRT-PCR结果表明NaCl胁迫不仅能够诱导大麦萌发期种子中HvMBF1c基因的表达,而且也能使苗期叶片及根系中该基因显著上调表达且叶片中的表达量明显高于根系,整体表达程度随种质耐盐能力及胁迫时间的增强而上升;表明HvMBF1c参与调控大麦不同生育期盐胁迫响应过程。

    Abstract:

    In order to decipher the potential function of barley salt-tolerant related gene HvMBF1c and its expression pattern under salt stress treatment condition, isolation of the HvMBF1c gene via homologous cloning method was performed in two barley germplasm resources ZY26 (salt-tolerant type) and ZY218 (salt-sensitive type), followed by bioinformatics analysis of the structure, physical and chemical properties, homologous protein differences and phylogeny. HvMBF1c contains a 471bp open reading frame found on barley chromosome 7HL without intron structure, and encodes 156 amino acids. The deduced protein contains an N-terminal MBF1 domain and a C-terminal HTH domain, with the characteristics of unstable, alkaline, hydrophilic, non-transmembrane and no signal peptide structure, and its secondary structure of α helix. The phylogenetic analysis showed that HvMBF1c was highly homologous to MBF1 of various plants, and was closely related to TaMBF1c of wheat. Subcellular localization indicated that the fusion protein was mainly located in the nucleus and cytoplasm. Under NaCl stress treatment condition, the induction on the expression of the HvMBF1c gene was observed in seeds, leaves and roots at seedling stage, while the expression level in leaves was significantly higher than that in roots. The overall expression level was correlating with the ability of barley germplasm salt tolerance and the time for stress treatment. Collectively, these results demonstrated that HvMBF1c was involved in regulating the response of barley to salt stress at different growth stages.

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  • 收稿日期:2021-11-22
  • 最后修改日期:2021-12-30
  • 录用日期:2022-01-14
  • 在线发布日期: 2022-01-19
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