甘薯冷胁迫转录组分析及ABA信号途径基因挖掘
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作者单位:

1.辽宁省农业科学院作物研究所;2.沈阳市薯类产学研联盟

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基金项目:

辽宁省农业科学院院长基金项目(2021QN2004);辽宁省农业科学院基本科研业务费计划项目(2021GR2927);农业部甘薯生物学与生物技术重点实验室课题(2021B231056)


Transcriptome Analysis and ABA Signal Transduction Pathway Related Genes Exploration in Sweetpotato under Low Temperature
Author:
Affiliation:

1.Crop Research Institute, Liaoning Academy of Agricultural Sciences;2.Shenyang Sweetpotato and Potato Industry-University-Research Alliance

Fund Project:

President''s Fund Project of Liaoning Academy of Agricultural Sciences(2021QN2004),Scientific Research Program of Liaoning Academy of Agricultural Sciences(2021GR2927),Key Laboratory of Sweet Potato Biology and Biotechnology, Ministry of Agriculture and Rural (2021B231056)

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    摘要:

    甘薯是世界第七大粮食作物,虽然甘薯对盐、旱等胁迫的抗逆性较强,但因其起源热带而对低温耐受性差,目前低温已经成为影响甘薯产量及限制甘薯种植面积的重要非生物胁迫。本研究以耐冷品种辽薯36为试验材料,在低温处理0 h、6 h、24 h和48 h后,利用转录组测序技术筛选与冷胁迫紧密相关的代谢通路和差异表达基因。结果共获得甘薯转录组数据75.01 Gb,与参考基因组比对效率达76.33%。差异表达基因分析结果表明,甘薯响应冷胁迫的过程中有6282 个基因上调表达,3881 个基因下调表达,其中283 个基因在低温处理6 h、24 h和48 h共有上调表达,26 个基因在三个处理中均下调表达。GO功能富集分析发现,差异表达基因被大量富集到蛋白激酶活性、过氧化物酶活性、氧化还原酶活性、蛋白质磷酸化、氨基酸跨膜转运、蛋白质生色团连锁及果胶分解代谢分子功能和生物学过程等分类条目中。KEGG代谢通路富集分析表明,苯丙烷生物合成、淀粉和蔗糖代谢、植物激素信号转导、植物-病原相互作用、谷胱甘肽代谢这5个代谢通路富集了大量的差异表达基因。本研究进一步筛选到ABA信号转导途径4 个关键基因(PYL、PP2C、SnRK2、ABF)与冷胁迫响应密切相关,为甘薯耐冷基因克隆及耐冷机制解析提供了理论依据。

    Abstract:

    Sweetpotato is the seventh important food crop in the world. Although sweet potato is widely adaptable to unfavorable conditions such as drought and high salt, this species originating from tropical region remains low-temperature sensitive. In this study, cold-tolerant sweet potato variety “Liaoshu 36” was subjected for cold-stress treatments at 0 h, 6 h, 24 h and 48 h, and total RNAs were sampled for the transcriptome sequencing analysis. The results showed that 75.01 Gb transcriptome data were produced, and 76.33% of them were mapped to the reference genome. The differentially expressed gene (DEG) analysis revealed 6282 up-regulated genes were and 3881 down-regulated genes. Co-expression patterns analysis showed that 283 genes were up-regulated and 26 genes were down-regulated at all three treatments (6 h, 24 h, and 48 h). GO enrichment analysis showed that DEGs were mainly enriched on seven categories: protein kinase activity, peroxidase activity, oxidoreductase activity, protein phosphorylation, amino acid transmembrane transport, protein-chromophore linkage and pectin catabolic process. KEGG pathway enrichment showed that DEGs involved in phenylpropanoid biosynthesis, starch and sucrose metabolism, plant hormone signal transduction, plant-pathogen interaction, glutathlone metabolism. Moreover, four key genes (PYL, PP2C, SnRK2 and ABF) in ABA signal transduction pathway were identified to be closely related to low temperature stress response, which provided theoretical basis for the cloning of sweet potato chilling tolerance genes and the analysis of cold tolerance mechanism.

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  • 收稿日期:2021-11-22
  • 最后修改日期:2021-12-16
  • 录用日期:2021-12-30
  • 在线发布日期: 2022-01-04
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