花生子仁糖代谢转录组-代谢组学联合分析
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1.华北作物改良与调控国家重点实验室/河北省作物种质资源实验室/河北农业大学;2.河北易园生态农业科技有限公司

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河北省高等学校科学技术研究项目(ZD2019051);河北省重点研发计划项目现代种业科技专项(19226363D);2021年度河北省保定市农业科技园区建设项目(2111N004)


Transcriptome-metabolome Combined Analysis of Kernel Sugar Metabolism in Peanut (Arachis hypogaea L.)
Author:
Affiliation:

1.State Key Laboratory of North China Crop Improvement and Regulation, Laboratory of Hebei Provincial Crop Germplasm Resources, Hebei Agricultural University;2.Hebei Yiyuan Ecological Agriculture Technology Co, Ltd

Fund Project:

Key Project of Science and Technology Research in Colleges and Universities of the Department of Education in Hebei Province (ZD2019051),Key Project of Science and Technology Research of Modern Seed Industry of the Department of S&T in Hebei Province (19226363D), Project of Agricultural Science and Technology Park Construction in Baoding City, Hebei Province (2111N004)

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    摘要:

    花生的营养保健价值不断受到重视,鲜食花生尤其是高糖甜花生逐渐被消费者喜爱。在花生品质育种中,子仁糖分已成为当今重要的育种目标之一,糖代谢分子调控机理的研究凸显重要。花生糖含量是花生品质性状的决定因子之一。本研究中的高糖花生品系DJ的含糖量和普通花生品系DG存在品种间显著差异。糖分含量测定结果表明,DJ子仁总糖、可溶性糖及蔗糖含量分别是DG的2.4倍(p-value = 8.38×10-12 < 0.001)、2.3倍(p-value = 3.64×10-10< 0.001)和4.0倍(p-value = 1.71×10-9 < 0.001)。RNA-seq及KEGG分析结果表明,3371个差异表达基因富集到117条通路,其中4条与糖代谢密切相关。筛选出与糖代谢相关的通路主干结构基因7个,包括SS、FBP、HK、MAN、PFK、GPI和TPSA;与衍生物相关的基因8个,包括USP、RHM 、GMLS、2个UGDH、UGE、GLCAK和GALE;新基因1个GAE。在差异代谢物中定位到蔗糖、果糖、海藻糖、1-磷酸甘露糖、腺苷二磷酸葡萄糖、二磷酸尿苷乙酰氨基葡萄糖、尿苷二磷酸葡萄糖等,其中蔗糖、果糖、海藻糖显著上调,差异倍数分别为1.18、1.33和8.37。转录组与代谢组联合分析结果表明,淀粉和蔗糖代谢与果糖和甘露糖代谢是糖代谢的关键通路,蔗糖、果糖和海藻糖为主要差异代谢物质。16个关键差异表达基因qRT-PCR验证结果表明,SS、HK和GPI上调,FBP、MAN、PFK、USP、RHM、TPSA、GMLS、2个UGDH、UGE、GLCAK、GALE和GAE下调,调控趋势与转录组学趋势一致。本研究结果将为深入探究花生子仁糖代谢的分子调控机理提供参考。

    Abstract:

    The nutritional and health value of peanuts has been paid more and more attention, and fresh peanuts, especially high-sugar sweet peanuts are favored by consumers. In peanut quality breeding, the sugar content of kernels has become one of the important targets, and the research on the molecular regulation mechanism of sugar metabolism is of great importance. Peanut sugar content is one of the determinants of peanut quality traits. The sugar content of the high-sugar peanut line DJ was significantly different from that of the conventional line DG. The total sugar, soluble sugar and sucrose content of DJ were 2.4 folds (p-value = 8.38×10-12 < 0.001), 2.3 folds (p-value = 3.64×10-10< 0.001) and 4.0 folds (p-value = 1.71×10-9 < 0.001) than that of DG, respectively. By RNA-seq and KEGG analysis, 3371 differentially expressed genes enriched in 117 pathways were identified, and four of them were closely related to glucose metabolism. Seven main regulation pathway related genes (including SS, FBP, HK, MAN, PFK, GPI and TPSA) and eight derivative related genes (including USP, RHM, GMLS, 2 UGDH, UGE, GLCAK and GALE) as well as a new gene GAE possibly related to sugar metabolism were detected. Out of the identified differential metabolites, sucrose, D-fructose, trehalose, D-mannose 1-phosphate, ADP-glucose, UDP-N-acetylglucosamine and uridine diphosphate glucose, three metabolites sucrose, fructose and trehalose were significantly up-regulated with the difference folds of 1.18, 1.33 and 8.37, respectively. Transcriptome and metabolome combined analysis showed that starch and sucrose metabolism and fructose and mannose metabolism were the key pathways. Sucrose, fructose and trehalose were the main differential metabolites. By qRT-PCR detection of 16 key differentially expressed genes, three genes SS, HK and GPI were up-regulated, while FBP, MAN, PFK, USP, RHM, TPSA, GMLS, 2UGDH, UGE, GLCAK, GALE and GAE were down-regulated, as revealed by the transcriptome analysis. The results of this study could provide reference for further exploring the molecular regulation mechanism of sugar metabolism in peanut.

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马钰聪,赵雨露,李佳伟,等.花生子仁糖代谢转录组-代谢组学联合分析[J].植物遗传资源学报,2022,23(4):1143-1154.

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  • 收稿日期:2021-11-21
  • 最后修改日期:2022-01-15
  • 录用日期:2022-03-25
  • 在线发布日期: 2022-07-08
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