沙柳 SpsLAZY1a、SpsLAZY1b 基因启动子克隆及表达分析
作者:
作者单位:

1.内蒙古农业大学林学院;2.鄂尔多斯市林业和草原事业发展中心;3.鄂尔多斯市造林总场

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金(31660216);内蒙古自治区应用技术研究与开发资金项目(2021GG0075,2019GG004);国家科技重大专项课 题(2018ZX08020002-005-005)


Cloning of SpsLAZY1a and SpsLAZY1b Gene Promoters from Salix psammophila and Their Expression Analysis
Author:
Affiliation:

1.Forestry College of Inner Mongolia Agricultural University;2.Ordos Forestry and Grassland Enterprise Development Center,Inner Mongolia Ordos;3.Ordos Afforestation Station, Inner Mongolia Ordos

Fund Project:

National Natural Science Foundation of China (31660216),Inner Mongolia Autonomous Region Applied Technology Research and Development Fund Project (2021GG0075,2019GG004),National Science and Technology Major Special Project (2018ZX08020002-005-005)

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    为研究 SpsLAZY1a、SpsLAZY1b 基因的表达调控机制,本研究从沙柳中分离了 SpsLAZY1a、SpsLAZY1b 基因的启 动子片段。使用 PlantCARE 数据库对该启动子片段中顺式作用元件预测显示,SpsLAZY1a 和 SpsLAZY1b 启动子序列中除 了包含核心元件 CAAT-box 和 TATA-box 外,还含有光、茉莉酸甲酯、脱落酸、低温、乙烯、赤霉素等响应元件。ProSpsLAZY1a 和 ProSpsLAZY1b 在烟草中瞬时表达和转基因 84K 杨中的 GUS 显色结果表明,ProSpsLAZY1a 和 ProSpsLAZY1b 具有启动子活性。对转基因 84K 杨的 GUS 酶活检测结果表明,ProSpsLAZY1a 的 GUS 表达活性强于 ProSpsLAZY1b。对转基因 84K 杨茎的切片结果显示,蓝色显 色反应主要集中于内皮层和韧皮部。上述结果表明,SpsLAZY1a、SpsLAZY1b 基因的启动子是非组织特异性表达启动子,并且 这两个基因启动子的活性存在差异。本研究结果为解析 LAZY 基因的上游调控机制提供参考。

    Abstract:

    To study the transcriptional regulation mechanism of SpsLAZY1a and SpsLAZY1b genes, their promoter fragments were cloned from Salix psammophila C. Wang & C. Y. Yang. By analyzing cisacting element in the promoter sequences using plantCARE database,the promoter sequences of both genes contain the core elements CAAT-box and TATA-box,and the elements responding to light,methyl jasmonate, abscisic acid,ethylene,gibberellin,and low temperature. GUS staining in tobacco transient expression and stable transgenic 84K poplar revealed the transcriptional activity of both promoters ProSpsLAZY1a and ProSpsLAZY1b. The ProSpsLAZY1a activity was stronger than that of ProSpsLAZY1b in transgenic 84K poplar. The results of stem basal sections of transgenic 84K poplar showed that the expression of ProSpsLAZY1a and ProSpsLAZY1b was mainly observed in endothelium and phloem. The promoters of SpsLAZY1a and SpsLAZY1b genes were non-tissue-specific,and their activities of the promoters were different. Collectively,this study provides a reference for analyzing the regulation mechanism of LAZY gene.

    参考文献
    相似文献
    引证文献
引用本文

刘 璐,贺玉娇,王佳琪,等.沙柳 SpsLAZY1a、SpsLAZY1b 基因启动子克隆及表达分析[J].植物遗传资源学报,2022,23(3):917-925.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2021-10-11
  • 最后修改日期:2021-12-27
  • 录用日期:2022-01-14
  • 在线发布日期: 2022-05-11
  • 出版日期:
您是第位访问者
京ICP备09069690号-5
植物遗传资源学报 ® 2022 版权所有
技术支持:北京勤云科技发展有限公司