小麦根系响应高盐胁迫的时序转录组分析
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作者单位:

1.济宁学院生命科学与生物工程学院;2.中国农业科学院作物科学研究所

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基金项目:

国家自然科学基金(31601302);国家重点研发计划项目(2016YFD0100304);中国农业科学院科技创新工程项目;江苏省现代作 物生产协同创新中心;现代作物生产省部共建协同创新中心


Time-course Transcriptome Analysis in Wheat Root in Response to High Salinity
Author:
Affiliation:

1.College of Life Science and Bioengineering,Jining University;2.Institute of Crop Science,Chinese Academy of Agricultural Sciences

Fund Project:

National Natural Science Foundation of China(31601302),National Key Research and Development Program of China (2016YFD0100304),Agricultural Science and Technology Program for Innovation Team,CAAS,Jiangsu Collaborative Innovation Center for Modern Crop Production,Collaborative Innovation Center for Modern Crop Production co-sponsored by Province and Ministry

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    摘要:

    小麦是重要的粮食作物,盐胁迫严重影响小麦的生长发育,小麦的耐盐机制尚不完全清楚。本研究以济麦 19 为材 料,采用 NaCl 处理和时序 RNA 测序技术(time course RNA-seq)分析小麦根系响应高盐胁迫的分子机制。与盐胁迫 0 h 相 比,不同处理时间下小麦根中响应盐胁迫的差异表达基因总数为 5526 个。通过 Gene Ontology(GO)和 Kyoto Encyclopedia of Genes and Genomes(KEGG)分析发现,在盐胁迫处理较早时期(2 h 与 6 h),差异表达基因在植物激素信号转导、氨基酸代 谢、次级代谢等类别中显著富集。盐胁迫处理 6 h 之后,参与逆境应答过程的差异表达基因开始富集。随着胁迫时间的延长, 对植物伤害加剧,与高分子配合物、DNA 构象变化、蛋白质-DNA 结构变化相关的差异表达基因在盐处理 48 h 和 72 h 时富 集。参与信号传导,抗氧化胁迫,渗透胁迫,离子平衡和氨基酸合成的多个基因在盐胁迫处理不同时期都有差异表达,其中后 三者多为盐胁迫诱导表达的差异表达基因。这些结果为小麦耐盐分子机制的研究提供了有价值的信息。

    Abstract:

    Wheat(Triticum aestivum L.)is an important food crop worldwide. However,its growth has been heavily suppressed by salt stress. The molecular mechanisms of salt tolerance in wheat remain unclear. In this study we conducted research to decipher the mechanism of salt stress response in Jimai19 using NaCl treatment and time-course RNA sequencing. The total number of differentially expressed genes(DEGs)in response to salt stress was 5526 in wheat roots. Based on Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes (KEGG)analysis,it was found that DEGs were significantly enriched in the categories of plant hormone signal transduction,primary metabolic processes such as amino acid synthesis,and some secondary metabolic processes in the early stages of salt treatment. After 6 h of salt treatment,the DEGs involved in stress response began to enrich. With the extension of stress time and the damage to plant increased,the DEGs related to macromolecular complex,DNA conformational changes,protein-DNA structural changes etc,were enriched at 48 h and 72 h of salt treatment. Many genes involved in signal transduction,resistance to oxidative stress,osmotic stress,ion balance and amino acid sythesis were differentially expressed at different stages of salt treatment. Most genes of the last three classes were induced by salt stress. These results provided valuable information on the salt tolerance of molecular mechanisms in wheat.

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李红燕,陈向前,牛凤娟,等.小麦根系响应高盐胁迫的时序转录组分析[J].植物遗传资源学报,2022,23(2):592-604.

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历史
  • 收稿日期:2021-09-27
  • 最后修改日期:2021-12-02
  • 录用日期:2021-12-14
  • 在线发布日期: 2022-03-09
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