1.Sanming Academy of Agricultural Sciences, Fujian Sanming;2.Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University
Fujian Provincial Science and Technology Plan Pilot Project (2018N0007, 2019N0004)，Fujian Science and Technology Foreign Cooperation Industrialization Project (2018I1011)
剖析红米抗氧化活性成分的配合力和遗传力，为富含功能营养成分的红米杂交水稻选育提供理论参考。选用6个三系不育系和8个红米恢复系，按不完全双列杂交6 × 8（NCII）设计，配制48个杂交红米组合，对总酚、总黄酮、原花青素和花色苷含量进行配合力及遗传参数分析。结果表明：红米杂交组合间的总酚、总黄酮、原花青素和花色苷含量存在遗传差异，受不育系亲本加性和组合间非加性效应共同影响，其中总酚、总黄酮和原花青素含量主要由基因非加性效应起主导作用，花色苷含量受基因加性效应和基因非加性效应互作的共同影响；亲本一般配合力（GCA）的高低与组合特殊配合力（SCA）的优劣之间无直接相关，不同糙米颜色的母本与所配红米组合的特殊配合力（SCA）也无明显相关性；遗传力分析表明，4个抗氧化活性成分的狭义遗传率变幅为12.99 %～48.29 %，宜在中、高世代选择有效；不育系的4个抗氧化活性成分贡献率均大于恢复系，红米杂交稻育种中要加强母本的选择；母本‘野香A’、‘品红1A’在红米抗氧化成分育种上有较好的实际利用价值，‘野香A’×‘18Rr175’和‘广8A’×‘18Rr178’两个杂交组合的特殊配合力（SCA）效应值高，生产应用潜力大。
This study analyzed the combining ability and heritability of the antioxidant active ingredients of red rice, which will provide theoretical reference for breeding of hybrid red rice rich in functional nutrients. By deployment of 6 three-line sterile lines and 8 red rice restorer lines to generate 48 hybrids of red rice following the incomplete diallel hybridization 6 × 8 (NCII), the combining ability and genetic parameters of total phenols, total flavonoids, procyanidins and anthocyanins were analyzed. The results showed that there were genetic differences in the content of total phenols, total flavonoids, procyanidins and anthocyanins among the red rice hybrid combinations, which were affected by the additive effects of the sterile line parents and the non-additive effects between the combinations. The contents of total phenols, total flavonoids and proanthocyanidins were mainly dominated by the non-additive effects of genes, and the content of anthocyanins was affected by the interaction of additive and non-additive effects of genes. There was no direct correlation between the parental general combining ability (GCA) and the combined special combining ability (SCA), and there was no obvious correlation between the female parent with different brown rice color and the special combining ability (SCA) of the red rice combination. The heritability analysis showed that the narrow-sense heritability of the four antioxidant active ingredients ranged from 12.99 %-48.29 %, which should be effective for selection in the middle and high generations. The contribution rate of the four antioxidant active ingredients of the sterile lines was greater than that of the restorer lines. The selection of female parents should be strengthened in the breeding of red rice hybrid rice. The female parents ‘Yexiang A’ and ‘Pinhong 1A’ were better in the breeding of red rice antioxidant components. Hybrid combinations ‘Yexiang A’ × ‘18Rr175’ and ‘Guang 8A’ × ‘18Rr178’ had high special combining ability (SCA) effect value and great potential for production and application.