小麦籽粒性状热感指数相关分子标记的鉴定及分析
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1河北工程大学园林与生态工程学院,邯郸 056000;2河北省农林科学院生物技术与食品科学研究所 / 河北省植物转基因中心重点实验室,石家庄 050051;3 石家庄市农林科学研究院,石家庄 050000

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河北省现代农业创新工程(494-0402-YBN-S2XB,494-0402-YBN-CTGQ);河北省重点研发计划项目(21326322D);石家庄市科学技术研究与发展规划(191490332A)


Identification and Analysis of Molecular Marker Related to Heat Sensitivity Indexes of Grain Traits in Wheat
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1 College of Landscape architecture and Ecological Engineering,Hebei University of Engineering,Handan 056000; 2 Institute of Biotechnology and Food Science,Hebei Academy of Agriculture and Forestry Sciences/ Key Laboratory of Plant Genetic Engineering Center of Hebei Province,Shijiazhuang 050051; 3 Shijiazhuang Academy of Agricultural and Forestry Sciences,Shijiazhuang 050000

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Technological Innovation Project of Modern Agriculture of Hebei Province (494-0402-YBN-S2XB,494-0402-YBN-CTGQ); Hebei Province Key Research and Development Plan Project (21326322D); Science and Technology Research and Development Programme of Shijiazhuang (191490332A)

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    摘要:

    小麦耐热性是多基因控制的复杂数量性状,通过关联耐热性状获得耐热分子标记,利用该标记辅助选择育种可提高育种的准确性,加快小麦耐热育种进程。本研究对190份小麦品种(品系)的千粒重、籽粒长度、籽粒宽度、籽粒表面积和籽粒周长5个籽粒相关性状的热感指数进行关联分析,鉴定出两个耐热相关分子标记Xwmc622、Xwmc707。聚丙烯酰胺凝胶电泳分析表明:Xwmc622、Xwmc707各自具有I、II、III三个等位变异类型。Xwmc622的I型等位位点与千粒重热感指数、籽粒宽度热感指数和籽粒周长热感指数呈极显著负相关关系(P<0.01),同时I型等位位点与籽粒长度热感指数和籽粒表面积热感指数呈显著负相关关系(P<0.05),I型小麦品种(品系)的耐热指数平均值小于1,为优良等位变异;II型等位位点与千粒重热感指数呈极显著正相关关系(P<0.01),与籽粒长度热感指数、籽粒表面积、籽粒周长呈显著正相关关系(P<0.05),II型等位变异小麦品种(品系)的热感指数平均值大于1;III型等位变异与小麦籽粒性状热感指数都不相关。Xwmc707的I型等位变异与小麦籽粒性状热感指数都不相关;II型等位位点与千粒重热感指数呈显著负相关关系(P<0.05),II型小麦品种(品系)的耐热指数平均值小于1,为优良等位变异;类型III的小麦材料与千粒重热感指数、籽粒宽度热感指数、籽粒周长热感指数呈显著正相关(P<0.05),III型等位变异小麦品种(品系)的热感指数平均值大于1。研究结果表明:Xwmc622的I等位变异为耐热优良等位变异;Xwmc707的II型等位变异为优良等位变异。这为小麦耐热分子标记辅助育种提供了参考信息。

    Abstract:

    Heat tolerance of wheat is a complex quantitative trait controlled by multiple genes. Molecular markers related to heat tolerance can be acquired by correlation analysis. With these tolerant markers,the accuracy of molecular marker-assisted selection breeding may be improved,and the process of breeding for heat tolerant wheat may be accelerated. In this study,heat sensitivity indexes (HI) of five grain traits,including 1000-kernel weight,kernel length,kernel width,kernel surface area,and kernel circumference were identified in 190 wheat varieties (lines). Molecular markers Xwmc622 and Xwmc707 related to heat sensitivity indexes were obtained by simple correlation analysis. Polyacrylamide gel electrophoresis analysis showed that Xwmc622 and Xwmc707 each had three allelic types: I,II and III. Type I allelic loci of Xwmc622 were extremely significantly negatively correlated with HI of 1000-kernel weight,of kernel width,and of kernel circumference (all P < 0.01),and significantly negatively correlated with HI of kernel length and of kernel surface area (all P < 0.05). The average values of HI in wheat varieties (lines) with type I allelic variation were less than 1. Type II allelic loci of Xwmc622 were extremely significantly positively correlated with HI of 1000-kernel weight (P < 0.01),and significantly positively correlated with HI of kernel length,of kernel surface area,and of kernel circumference (all P < 0.05). The average values of HI in wheat varieties (lines) with type II allelic variation were greater than 1. Type III allelic loci of Xwmc622 were not correlated with HI of grain traits. Type I allelic loci of Xwmc707 were not correlated with HI of wheat grain traits. Type II allelic loci of Xwmc707 were significantly negatively correlated with HI of 1000-kernel (P < 0.05). The average values of HI in wheat varieties (lines) with type II allelic variation were less than 1. Type III allelic loci of Xwmc707 were significantly positively correlated with HI of 1000-kernel weight,of kernel width,and of kernel circumference (all P < 0.05). The average values of HI of the grain traits in wheat varieties (lines) with type III allelic variation were greater than 1. The results showed that allele I of Xwmc622 and allele II of Xwmc707 were superior alleles of wheat heat tolerance,which provided reference information for molecular marker-assisted breeding for wheat heat resistance.

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邵雪飞,李国良,杨阳,等.小麦籽粒性状热感指数相关分子标记的鉴定及分析[J].植物遗传资源学报,2021,22(6):1635-1643.

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  • 收稿日期:2021-04-15
  • 最后修改日期:2021-08-12
  • 录用日期:2021-09-07
  • 在线发布日期: 2021-11-04
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