藜麦种质资源遗传多样性SSR标记分析
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1.成都大学农业农村部杂粮加工重点实验室;2.中国农业科学院作物科学研究所;3.伊犁哈萨克自治州农业科学研究所;4.东北师范大学草地科学研究所/植被生态科学教育部重点实验室

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中国农业科学院科技创新工程专项经费(CAAS-ASTIP-2017-ICS)


Genetic Diversity Analysis of Quinoa by SSR Markers
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1.Chengdu University Key Laboratory of Minor Cereal Processing, Ministry of Agriculture and Rural Affairs;2.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences;3.Institute of Agricultural Sciences of Ili Kazak Autonomous Prefecture;4.Ministry of Education Key Laboratory of Vegetation Ecology / Institute of Grassland Sciences, Northeast Normal University

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    摘要:

    为研究藜麦(Chenopodium quinoa Willd.)种质资源的遗传多样性,分析国内藜麦种质遗传背景,本研究利用66个简单重复序列(SSR)标记对163份藜麦种质和3份台湾红藜(Chenopodium formosanum Koidz.)种质进行分子标记,分析了该种质群体的多态性和亲缘关系。数据显示,66对SSR标记在166份种质材料中检测到327个等位位点,平均每个标记5.031个等位位点,平均观测杂合度和期望杂合度分别为0.387和0.588,平均多态性信息含量为0.524。用类平均法将166份材料聚为3个组群,其中Ⅰ组仅包括3份台湾红藜,Ⅱ组包括以来源于美国国家种质库和智利种质为主的103份种质材料,Ⅲ组包括以来源于玻利维亚和秘鲁种质为主的60份种质材料。群体结构分析和主成分分析将藜麦群体划分为两个亚群,亚群之间有基因交流。结果表明,玻利维亚和秘鲁种质与美国和智利种质的遗传信息存在明显区分,来自青海和云南的藜麦种质在亲缘关系上更接近安第斯高原型,来自河北、山西的藜麦种质更接近智利低海拔型。台湾红藜为台湾本土种质。

    Abstract:

    In order to study the genetic diversity and the genetic background of quinoa (Chenopodium quinoa Willd.) germplasms distributed in China, molecular markers research was carried out based on 163 quinoa accessions and 3 djulis (Chenopodium formosanum Koidz.) accessions by using 66 simple repeat sequence (SSR) markers, to analyze the polymorphism and inter-species relationship. According to the data, a total of 327 alleles were amplified from 66 SSR markers, with an average of 5.031 alleles per marker. The mean values of observed and expected heterozygosity were 0.387 and 0.588, respectively, while the mean values of the polymorphism information index was 0.524. The method of UPGMA clustered all the accessions into three groups. Group I included 3 djulis accessions only. Group II included 103 accessions, most of which were derived from USDA-NPGS and Chilean types. Group III included 60 accessions, most of which were derived from Bolivian and Peru types. The quinoa population was divided into two groups by population structure analysis and principal component analysis, and there was gene exchange between the two groups. The results indicate that there is a clear distinction between Bolivian, Peru accessions and American, Chilean accessions in genetic information, and the genetic relationship of the accessions from Qinghai and Yunnan are closer to Andean highland type, while the accessions from Hebei and Shanxi are closer to Chilean lowland type. Djulis is a native plant in Taiwan.

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孙梦涵,邢宝,崔宏亮,等.藜麦种质资源遗传多样性SSR标记分析[J].植物遗传资源学报,2021,22(3):625-637.

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  • 收稿日期:2020-09-11
  • 最后修改日期:2020-10-10
  • 录用日期:2020-10-12
  • 在线发布日期: 2021-05-07
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