渝17S对低温胁迫的响应及耐冷机制初步研究
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1 重庆市农业科学院生物技术研究中心 / 逆境农业研究重庆市重点实验室,重庆 401329; 2 重庆市农业科学院重庆再生稻研究中心,重庆 402160

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重庆市农业科学院青年创新团队项目(NKY-2018QC04)


The Effect of Low Temperature Stress on the Growth of‘Yu17S’and a Preliminary Study of Cold-resistance Mechanisms
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1Biotechnology Research Center, Chongqing Academy of Agricultural Sciences/Chongqing Key Laboratory of Adversity Agriculture, Chongqing 401329; 2Chongqing Ratooning Rice Research Center, Chongqing Academy of Agricultural Sciences, Chongqing 402160

Fund Project:

Program for Young Innovative Research Team in Chongqing Academy of Agricultural Sciences(NKY-2018QC04)

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    摘要:

    重庆及西南稻区水稻幼苗期倒春寒频发,常常导致秧苗生长迟缓,白化、烂秧等发生,制约了优质水稻安全生产和 轻简化技术的应用。本研究以耐冷性较强的渝 17S 和对低温敏感的 Y58S 为材料,对比研究了 3 叶龄幼苗经 4 ℃低温处理 后,二者可溶性糖、海藻糖、脯氨酸、丙二醛和过氧化氢的含量,超氧化物歧化酶、过氧化氢酶和抗坏血酸过氧化物酶的活性, 以及荧光定量 PCR 检测胁迫相关的 9 个基因的表达情况。结果表明:与 Y58S 相比,渝 17S 具有更强的苗期耐冷性,在低温 胁迫下,其死苗率为 7.5%,而 Y58S 死苗率高达 96.7%;低温胁迫下 2 个品种叶片中丙二醛和 H2O2 含量均升高,但渝 17S 增 幅较小;渝 17S 叶片中抗氧化酶( 超氧化物歧化酶、过氧化氢酶和抗坏血酸过氧化物酶)活性,可溶性糖和海藻糖含量,以及 OsCATB、 OsSOD、 OsAPx8, OsTPS1、 OsTPP1、 OsMKK6、 OsMAPK3 和 OsICE1 基因表达量均呈不同程度增加; 2 个品种叶片 中脯氨酸含量和脯氨酸合成限速酶基因( OsP5CS)的表达量未见明显差异。初步认为, H2O2 信号分子和海藻糖可以调控渝 17S 对低温胁迫的响应,从而增强其幼苗的抗寒性。

    Abstract:

    “Late spring coldness” negatively affects rice seedling establishment and leads to rice growth retardation, albinism and rotten in Chongqing and southwest China, which severely restricts the production of high-quality rice and the application of optimal cultivation technologies. To investigate the cold tolerance of the chilling-tolerant indica cultivar Yu17S and chilling-sensitive indica cultivar Y58S seedlings, different parameters including the contents of soluble sugar, trehalose, proline, MDA and H2O2, the activities of antioxidant enzyme( SOD, POD and CAT), and the relative expression of stress relative genes were deployed. If compared with Y58S with the dead seedlings rate of up to 96.7%, Yu17S represented 7.5% of the dead seedlings rate, suggesting a stronger cold tolerance at the seedling stage upon the low temperature stress. The contents of both H2O2 and MDA increased in both cultivars under the low temperature stress, whereas both values of Yu17S were lower than these of Y58S. In Yu17S, the CAT, SOD and APX activity, soluble sugar and trehalose content, as well as the transcripts of genes OsCATB, OsSOD, OsAPx8, OsTPS1, OsTPP1, OsMKK6, OsMAPK3 and OsICE1 have been observed to be significantly increased. No significant differences on the proline content and on the expression of OsP5CS that controls the proline biosynthesis in both cultivars were observed. It is possible that the H2O2 signal molecule and trehalose of Yu17S may respond to the low temperature stress at the seedling stage, thus resulting in an enhancement on cold resistance.

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潘晓雪,张现伟,李经勇,等.渝17S对低温胁迫的响应及耐冷机制初步研究[J].植物遗传资源学报,2021,22(1):205-213.

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  • 收稿日期:2020-05-27
  • 最后修改日期:2020-07-23
  • 录用日期:2020-08-24
  • 在线发布日期: 2021-01-07
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