63份谷子种质的耐盐综合评价及耐盐品种筛选
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山东农业大学 资源与环境学院,山东农业大学 资源与环境学院,山东农业大学 资源与环境学院,山东农业大学 资源与环境学院,山东农业大学 资源与环境学院,山东农业大学 资源与环境学院,泰安高新技术产业开发区 农业工作办公室

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山东省现代农业产业技术体系杂粮创新团队栽培与土壤肥料岗位专家经费(SDAIT-15-04);山东省自主创新及成果转化专项(2014ZZCX07402);山东省重点研发计划(产业关键技术)(2016CYJS05A02);山东省博士后创新项目(201603052)。


Comprehensive Evaluation of Salt Tolerance and Screening for Salt Tolerant Accessions of 63 Foxtail Millet Germplasm
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College of Resources and Environment of Shandong Agricultural University,College of Resources and Environment of Shandong Agricultural University;,College of Resources and Environment of Shandong Agricultural University,College of Resources and Environment of Shandong Agricultural University,College of Resources and Environment of Shandong Agricultural University,College of Resources and Environment of Shandong Agricultural University,

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    摘要:

    谷子是我国北方重要的粮食作物,具有较强的耐盐抗旱能力。盐碱地是我国重要的后备耕地资源,加强谷子种质耐盐评价、筛选耐盐碱的谷子品种对促进盐碱地资源利用、保障国家粮食安全具有非常重要的作用。本试验以63份谷子种质资源为研究对象,以1.0%质量浓度NaCl溶液进行盐胁迫,用蒸馏水作为对照,采用全营养培养基发芽法在人工气候培养箱(昼/夜,30℃/20℃,光周期14h/d)中进行谷子种质萌发期的耐盐性鉴定;利用相关性分析和主成分分析明确8项耐盐指标在耐盐性评价中的权重;通过模糊数学中的隶属函数法和聚类分析法对63份谷子种质的耐盐性进行综合评价和耐盐等级划分。结果表明:谷子种质资源的耐盐性存在广泛的变异;1.0%质量浓度的盐胁迫下,谷子的发芽率、发芽指数、根长、芽长、相对芽长、相对根长之间互成显著正相关关系,而与相对盐害率成显著负相关;主成分分析结果表明,发芽率、发芽指数、相对芽长、相对根长可作为谷子耐盐性评价的关键指标;通过聚类分析可将63份谷子种质分为极度耐盐种质、耐盐种质、盐敏感种质和极度盐敏感种质4类;基于模糊数学中的隶属函数得分进行综合评价,筛选获得了济谷16、汾特11号、峰红谷、晋谷57、晋汾106号等五份耐盐谷子品种。

    Abstract:

    Foxtail millet with strong salt resistance and drought resistance was recognized as an important grain crop in North China. It played vital important role in ensuring the food security in arid and poor areas, especially in the adjustment and optimization of agricultural planting structure in saline and alkaline soil which is an important reserve cultivated land resource in China.In present study, 63 accessions of foxtail millet were employed as experimental material, and were cultivated in total nutrient germination medium that exposed to 1.0% salinity regimes for salt stress identification at germination stage. Deionized water without salinity was used as control, and kept in artificial climate incubator(day/night,30℃/20℃,photoperiod 14h/d) . Correlation analysis and principal component analysis were employed to evaluate the role of eight salt tolerance-related indexes. Membership function analysis and cluster analysis were also evaluated to identify the grade of salt tolerance of foxtail millet germplasm. The results showed that: There is wide variation of salt tolerance in foxtail millet germplasm resources. Under 1% concentration of salt, the germination rate, germination index, root length, germinal length, relative germinal length and relative root length of plant were positively correlated with each other, and the relative salt harm rate has a significant negative correlation with the 6 indexes. According to principal component analysis, germination rate percentage, germination index, relative germinal length and relative root length were used as indexes to evaluate salt tolerance of foxtail millet; 63 millet germplasm are divided into extreme salt tolerant germplasm, salt tolerant germplasm, salt sensitive germplasm, extreme salt sensitive germplasm with cluster analysis. Therefore, Fenghonggu, Jinfen 106, Jingu57, Fente11, Jigu 16 was selected as relative salt tolerant germplasm with comprehensive evaluation of membership function score based on Fuzzy Mathematics.

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韩飞,诸葛玉平,娄燕宏,等.63份谷子种质的耐盐综合评价及耐盐品种筛选[J].植物遗传资源学报,2018,19(4):685-693.

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  • 收稿日期:2017-11-07
  • 最后修改日期:2018-04-03
  • 录用日期:2018-01-12
  • 在线发布日期: 2018-07-16
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