黄秋葵类胡萝卜素合成基因LCYB的克隆与分析
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

福建省属公益类科研院所基本科研专项(2015R1026-9);福建省自然科学(2015J01118);福建省农业科学院创新团队PI项目(2016PI-40)通信作者 Author for correspondence(E-mail:zhs0246@163.com;fjvrc@163.com)


Cloning and Expression Analysis of a Lycopene Beta-cyclase Gene in Hibiscus esculentus L
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    本研究应用RT-PCR和RACE技术克隆得到cDNA全长1797 bp的黄秋葵番茄红素β-环化酶基因LCYB,开放阅读框(ORF) 1509个碱基;预测编码503个AA,理论分子量(Mw)为56.288 kD,等电点(pI)为4.577;编码的蛋白与陆地棉(Gossypium raimondii)、黄麻(Corchorus olitorius)和可可(Theobroma cacao)同源蛋白的相似性均在88%以上,显示其高度的保守性,将基因命名为HyLCYB,GeneBank登录号为:KX257998。Motif Scan分析显示,蛋白氨基酸序列88 ~ 481位为HyLCYB保守结构域,并在88 ~ 113位含有1个FAD结合域。通过荧光定量PCR 分析表明,LCYB基因在黄秋葵根、茎、叶、花和果荚中都有表达;叶片生长中以成熟叶中表达最高,果实发育中以花后7天高表达。建立与优化了黄秋葵类胡萝卜素超高效液相检测体系,黄秋葵中主要含有β-胡萝卜素和叶黄素。β-胡萝卜素在成熟叶中含量最高, 果实以花后7天的果实含量最高,与LCYB基因的表达存在一定的相关性。

    Abstract:

    In this study, a LCYB gene was cloned from Hibiscus esculentus L. by RT-PCR and RACE technique. The full-length cDNA sequence of LCYB was 1797 bp, which contained a 1509 bp open reading frame(ORF)that encoded 503 amino acids,with a predicted molecular weight (mol.wt.) of 56.288 kD and a hypothetical isoelectric point (pI) of 4.577. It shared over88% identity with the homologous proteins from Gossypium raimondii, Corchorus olitoriu. and Theobroma cacao, proving that it was highly conservative. This gene was named HyLCYB and the GeneBank accession was KX257998. Motif Scan analysis showed that HyLCYB protein had a conservative structure domain and binding domain in the position of 88-481 and 88-113 sites,respectively. Real-time PCR analysis revealed that LCYB could be expressed in different tissues of Hibiscus esculentus L., including roots, stems, leaves, flowers and fruits. The expression level of LCYB gene was the highest in mature leaves and 7 days after flowering during the process of Leaf and fruit development respectively. What’s more, an ultra-high performance liquid chromatography (UPLC) method for the determination of carotenoid contents was explored. The carotenoids in Hibiscus esculentus L. primarily consisted of lutein and β-carotene, andβ-carotene contents was also the highest in mature leaves and 7 days after flowering during the process of Leaf and fruit development respectively, which probably related to the changes in LCYB genes expression.

    参考文献
    相似文献
    引证文献
引用本文

李永平,陈敏氡,朱海生,等.黄秋葵类胡萝卜素合成基因LCYB的克隆与分析[J].植物遗传资源学报,2018,19(1):167-176.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2017-04-12
  • 最后修改日期:2017-07-11
  • 录用日期:2017-07-11
  • 在线发布日期: 2018-01-23
  • 出版日期:
您是第位访问者
ICP:京ICP备09069690号-23
京ICP备09069690号-23
植物遗传资源学报 ® 2024 版权所有
技术支持:北京勤云科技发展有限公司