天津和唐山小豆地方品种遗传多样性分析
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国家食用豆行业科研(nyhyzx07-017),国家食用豆产业技术体系建设(CARS-09)


Analysis of the Genetic Diversity of Adzuki Bean Native Variety
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    摘要:

    利用SSR分子标记对唐山红小豆、天津红小豆的96个品种(按品种地地理来源划分为14个品种群)进行遗传多样性分析,表明197对引物中共有89对的扩增谱带清晰稳定,共扩增出285条带,多态性条带百分率(PPB)达100%,经Popgene32软件分析,遵化品种资源的遗传多样性的水平最高[PPB=85.39%,I(信息指数)=0.567],其次为玉田和迁安的品种资源,而在天津红小豆资源中,除静海县(排位第5)外,遗传多样性水平相对较小,其中宁河的遗传多样性水平最低(PPB=22.47%,I=0.1575)。聚类分析表明,96个品种可分为三个组群,唐山红小豆组群中包含了天津静海小豆;天津红小豆中的大港品种资源单独为一组群;其它天津红小豆为第二大组群。本研究可为地方品种的保存及杂交组配提供参考。

    Abstract:

    The genetic diversity of 96 landraces of Tangshan and Tianjin auzuki bean was investigated by SSR molecular markers . These materials can be divided into 14 populations in terms of their geographical origins. 89 out of 197 pairs of primers were detected producing 285 bands , with total percentage of polymorphic bands (PPB) was 100%. The genetic diversity of ‘Zunhua’ population was the highest and that of Yutian and Qian’an was second higest. All landraces from Tianjin excluding Jinghai population has relatively low genetic diversity and the lowest PPB with 22.47% can be found in Ninghe polpulation. Total of 96 accessions are divided into three groups by genetic similarities. Jinghai population from Tianjin was included by Tangshan auzuki bean and Dagang population from Tianjin was one separate group. The rest accessions of Tianjin auzuki bean froms another group. So reference for landraces conservation and selection of diverse parental combinations can be provided by this research.

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刘振兴.天津和唐山小豆地方品种遗传多样性分析[J].植物遗传资源学报,2011,12(5):679-685.

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  • 收稿日期:2011-05-19
  • 最后修改日期:2011-05-26
  • 录用日期:2011-06-07
  • 在线发布日期: 2011-07-22
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