矮牵牛PMADS9基因启动子的克隆及分析
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国家自然科学基金项目(30671481)


Cloning and Characterization of the PMADS9 Gene Promoter from Petunia
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    摘要:

    矮牵牛PMADS9基因是MADS-box基因AGL15亚家族的成员。该亚家族基因可能具有调控开花时间、抑制花器官衰老脱落和促进体胚形成等功能。本文应用YADE和hiTAIL-PCR等方法,克隆了PMADS9基因5′端翻译起始位点上游1853bp的启动子区域序列(FJ798977); RACE分析发现该基因至少有4个转录起始位点,2个位于编码区第一外显子内。启动子调控元件分析显示,PMADS9启动子富集花粉和种子发育过程中特异表达元件和与环境应答相关的元件;AGL15同源基因启动子存在非常保守的RY-repeat元件,启动子的保守性与物种的遗传距离不一致;推测PMADS9启动子翻译起始位点上游200~400bp和800~1000bp区域具重要功能。

    Abstract:

    The PMADS9 is a member of the AGL15 subfamily of MADS-box genes. The Arabidopsis AGL15 subfamily genes have been reported to act as inhibiters of flower senescence, repressors of floral transition and enhancers of somatic embryo development. Using YADE method and hiTAIL-PCR, we isolated 1853bp sequences upstream of the putative translation start of the PMADS9 gene from petunia. The results of 5′RLM-RACE analysis showed that the PMADS9 gene has at least 4 TSSs, and 2 of them are located in the first exon. Cis-regulatory elements of the PMADS9 promoter predicted by PLACE and PlantCARE are related with seed and pollen development and environmental response. Analysis of promoter sequences from AGL15-clade MADS-box genes by FootPrinter showed that there are very conserved RY-repeat motifs among them, and the conversation of promoters between Solanaceae and the selected 5 species of Rosids is higher than that between Brassicaceae and the same selected species,even though Solanaceae is less closely related to Rosids than Brassicaceae. Furthermore, the results also suggested that regions of 200-400bp and 800-1000bp upstream of the ATG were functionally important.

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郭余龙,闫明旭,陈君,等.矮牵牛PMADS9基因启动子的克隆及分析[J].植物遗传资源学报,2011,12(2):275-280.

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