This paper is devoted to identification and analysis of 20 new flue-cured tobacco germplasm resources, which have been newly collected, introduced, bred and catalogued in the National Bank of Tobacco Germplasm Resources. The results show that the varieties of Yunyan 317, Yunyan 201, Yunyan 202, Yunyan 203, PVH09 and RGH51, identified by National Committee of Tobacco Variety, have resistance or mid-resistance for more than 2 or more main diseases, such as black shank, tobacco root knot nematode, bacterial wilt, TMV etc., and have moderate yields as well as good quality. Baihuadajunyuan, Jiangchuan Seed, Dayou Seed and Renminliudui-15 have heavy susceptible to black shank. The rest of germplasm resources grow normally in the field. The related data materials, such as period of duration, morphological characteristics, main agronomic traits, leaf face quality, chemical components and main diseases, have been obtained through field experiments, and the photos of plant, leaf, inflorescence, corolla and capsule also been sent to Tobacco Research Institute of China Academy of Agricultural Sciences, so as to make contributions to the construction as well as allocation for National Information System of Tobacco Germplasm, enrich the resources of the National Bank of Tobacco Germplasm Resources, and make the continuous ultilization of germplasm resources for future research works.