分子标记在啤酒花种质资源研究中的应用
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甘肃省科技厅星火科技“啤酒花新品种亚金I号产业化开发”项目(0910HCNA074)


Molecular markers and their applications in hop germplasm
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    摘要:

    该论文利用分子生物学中常用的DNA分子标记对世界各地现存的野生和栽培的啤酒花种质资源遗传多样性研究的应用进展做一综述。通过查阅和研读20世纪90年代以来发表的各类文献进行归纳总结。发现DNA分子标记相比形态学标记和细胞学标记具有结果准确、稳定的特点,常用的分子标记技术有RAPD、RFLP、ISSR、SSR、AFLP、EST等;研究发现北美洲的啤酒花遗传多样性要高于欧洲的啤酒花,基因变异程度也相对较高;野生啤酒花的基因序列具有丰富的基因多样性,可在分子杂交遗传育种中作为一个种质去改善栽培品种的某些不良性状。因此,利用分子标记研究啤酒花的遗传多样性将对啤酒花的优良育种提供理论指导和技术支持,目前较为理想的技术是SSR和AFLP。

    Abstract:

    The review focused on the use of molecular biology of DNA molecular markers in the existing wild and cultivated hops of Genetic Diversity progress. Materials were summarized through reading all relative literatures published since 1990s. The results of DNA molecular markers technology are accurate and stable compared to morphological markers and cytological markers. This method is simple, rapid and requirement of sample is low. Random Amplified Polymorphism DNA (RAPD), Restriction Fragment Length Polymorphism (RFLP), Inter-simple Sequence Repeat (ISSR), Simple Sequence Repeat (SSR) and Amplified Fragment Length Polymorphism (AFLP) are commonly used molecular markers. We found genetic diversity is often higher in hops from North America than which from Europe. The same results were also found in the degree of genetic variation. Meanwhile, the wild hops' gene order has the rich gene diversity, may take an idioplasm in the molecular heredity hybridization breeding to improve the cultivated varieties’ some certain bad characters. However, molecular markers also have disadvantages that employees are required special skills and the cost is high. The widely used molecular markers in plant genetic diversity research solved a number of difficult issues that traditional method cannot deal with. The use of molecular markers in the study of genetic diversity of hops will be an excellent tool to provide theoretical guidance and technological support for the breeding of hops.

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杨晓翠.分子标记在啤酒花种质资源研究中的应用[J].植物遗传资源学报,2011,12(5):732-737.

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  • 收稿日期:2010-07-11
  • 最后修改日期:2011-06-03
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  • 在线发布日期: 2011-08-09
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