大豆种质对SMV成株和种粒斑驳抗性的SSR标记辅助鉴定
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国家重点基础研究发展计划(973计划)项目(2004CB117203-5);引进国际先进农业科学技术计划(948计划)项目(2006-G1(A));国家高技术研究发展计划(863计划)项目(2006AA100104-3


Identification of the SMV adult-plant and seed coat mottling resistance in soybean germplasms using SSR markers
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    摘要:

    对186份大豆种质资源进行了成株和种粒斑驳抗性鉴定,并利用与成株抗性及种粒斑驳抗性分别相关的SSR标记验证抗病毒分子辅助选择的可行性。结果表明:接种SMV1,选出成株和种粒双抗种质38份;成株抗病种质149份;种粒抗病种质45份;成株和种粒双感种质26份。利用与成株抗性相关的SSR标记Sat_229、Sat_317、Satt335、Satt160、Satt516、Sat_309进行检测,抗病毒资源筛选的准确率分别达到68.9%、74.3%、71.1%、69.8%、77.4%和68.2%;利用与种粒斑驳抗性相关的SSR标记Sat_297、 Sat_229、Sat_317、Satt335、Sct_188、Satt160、Satt516、Sat_133进行检测,Sat_317标记达79.1%,标记Sat_229、Satt335、Satt516和Sat_133抗病毒资源筛选的准确率均达70%以上,可以用作抗病毒分子辅助育种的选择标记。

    Abstract:

    One hundred and eighty six soybean germplasms were screened for resistance to adult-plant and seed coat mottling by the inoculation of strain SMV1, and the coincident rate between adult-plant resistance, seed coat mottling resistance to SMV and linkage SSR markers were evaluated. The results showed that among these 186 germplasms, 38 were resistant to both adult-plant and seed coat mottling; 149 were only resistant to adult-plant; 45 were only resistant to seed coat mottling; And 26 were both susceptible to adult-plant and seed coat mottling. 6 SSR markers, Sat_229, Sat_317, Satt335, Satt160, Satt516 and Sat_309, related to SMV adult-plant resistance, were used to identify the 186 soybean germplasms. The coincident rates between the SSR alleles and the resistant presence were 68.9%, 74.3%, 71.1%, 69.8%, 77.4% and 68.2%, respectively; 8 SSR markers, Sat_297, Sat_229, Sat_317, Satt335, Sct_188, Satt160, Satt516 and Sat_133, related to SMV seed coat mottling resistance, were used to identify the 186 soybean germplasms, the coincident rates of Sat_317 alleles with the resistant presence were 79.1%, and that of Sat_229, Satt335, Satt516 and Sat_133 were all over 70%. These markers related to SMV1 resistance were available for the marker assisted selection for SMV resistance breeding.

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李文福,朱晓双,王晓锋,等.大豆种质对SMV成株和种粒斑驳抗性的SSR标记辅助鉴定[J].植物遗传资源学报,2010,11(2):239-243.

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