水稻恢复系M630稻瘟病抗性改良及其代谢组研究
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安徽省农业科学院水稻研究所/安徽省水稻遗传育种重点实验室

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基金项目:

安徽省重点研发计划(912167100062);国家重点研发计划(2016YFD0100101-06,2017YFD0100406);国家自然科学基金(31801331,31901529);安徽省科技重大专项(18030701175);安徽省自然科学基金(2008085QC120)


Genetic Improvement and of Rice Blast Resistance in Restorer Line M630 and Metabolomic Analysis
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Rice Research Institute, Anhui Academy of Agricultural Sciences/Key Laboratory for Rice Genetics and Breeding of Anhui Province, Hefei, 230031

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The Key R&D Program of Anhui Province (912167100062); The National Key R&D Program of China (2016YFD0100101-06 and 2017YFD0100406); The National Natural Science Foundation of China (31801331 and 31901529); the Major Scientific Technological Project of Anhui Province (18030701175); Natural Science Foundation of Anhui Province (2008085QC120)

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    摘要:

    稻瘟病是水稻的主要病害之一,改良水稻稻瘟病抗性对水稻生产、推广具有重要意义。本研究以携带广谱抗稻瘟病基因Pi9材料9311-Pi9为供体,水稻优良恢复系M630为受体,将杂交、回交与分子标记辅助选择和背景筛选相结合,获得改良恢复系M630-Pi9。主要农艺性状和稻米品质分析显示改良后的M630-Pi9及其杂交组合徽两优630-Pi9与M630和徽两优630相比无显著差异。进一步利用安徽省优势混合生理小种进行苗期稻瘟病抗性鉴定,结果表明改良后的M630-Pi9相对于M630抗性明显增强;稻瘟病抗性自然病圃鉴定结果发现改良后的恢复系及其杂交组合抗性显著增加。为了更好地了解稻瘟病抗性基因对植株发育的代谢机制,对M630-Pi9和M630受稻瘟病病原菌侵染后的植株进行代谢组分析,结果显示与M630相比,M630-Pi9中有212种代谢物合成受到调控,其中155种含量降低,57种含量增加;与细胞壁有关的物质含量明显增加,对生物体具有毒害和免疫作用的生物碱类代谢物含量显著降低,与生物胁迫相关的黄酮类物质受到不同程度地调控。这些数据表明稻瘟病抗性基因Pi9可能通过调控植物体中代谢物质的变化进而抵御病原体的侵害。创制的新恢复系稻瘟病抗性显著提高,对稻瘟病抗性机制研究提供依据,为水稻抗病、高产育种提供新的种质。

    Abstract:

    Rice blast is one of the main diseases in rice, and improvement of rice blast resistance is of great significance in rice breeding. In this study, we constructed a new restorer line M630-Pi9 by making crosses in the rice variety 9311-Pi9 that carries the broad-spectrum blast resistance gene Pi9 with the excellent rice restorer line M630, followed by backcrossing combined with molecular marker-assisted selection and background detection. No significant difference on agronomic traits and rice quality was observed in M630-Pi9 and its hybrid Huiliangyou 630-Pi9 if relative to M630 and Huiliangyou 630, respectively. By test for rice blast resistance at the seeding stage was conducted in M630-Pi9 and M630 by using a mixture of predominant isolates from Anhui province, M630-Pi9 represented improved resistance than that of M630. Natural inoculation showed that the improved restorer lines and derived hybrids had significantly improved rice blast resistance. Moreover, a metabolomic analysis was conducted in order to identify the metabolites involved in rice blast resistance. 212 metabolites in M630-Pi9 compared with M630 were found, including 155 down-regulated and 57 up-regulated components. The contents of substances annotated for cell wall and alkaloids which have toxic or immunizing effects on the organism are significantly increased and decreased, respectively. The contents of flavonoids involved in adverse biological process have been modified, implying that the rice blast resistance gene Pi9 trade off the invasion of pathogens via possibly regulating the contents of metabolites. Collectively, this work provided an example of deciphering the mechanism of rice blast resistance and producing new germplasm for rice breeding.

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周坤能,夏加发,王元垒,等.水稻恢复系M630稻瘟病抗性改良及其代谢组研究[J].植物遗传资源学报,2021,22(2):427-437.

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  • 收稿日期:2020-08-04
  • 最后修改日期:2020-08-21
  • 录用日期:2020-10-09
  • 在线发布日期: 2021-03-09
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