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赵亚梅1,陈生煜1,游 乐1,韩 茹1,翟俊文1,任 惠2,吴沙沙1
(1福建农林大学园林学院/兰科植物保护与利用国家林业和草原局重点实验室, 福州 350002;2广西壮族自治区农业科学院园艺研究所, 南宁 530007)
关键词:  阳桃  木质素  结构基因  转录因子  表达模式
Identification and Analysis Lignin Biosynthesis Genes of Averrhoa carambola
ZHAO Ya-mei1, CHEN Sheng-yu1, YOU Le1, HAN Ru1, ZHAI Jun-wen1, REN Hui2, WU Sha-sha1
(1College of Landscape Architecture, Fujian Agriculture and Forestry University/Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, Fuzhou 350002; 2Horticulture Research Insistute of Guangxi Academy of Agricultural Science, Nanning 530007)
Lignin is the key component in the secondary cell wall of plants, which provides mechanical support and promotes water transport as well as has important impact responding to various biological and abiotic stresses. Averrhoa carambola L. is an important economic and ornamental species, the growing zone of which is restricted by its sensitivity to environmental stress conditions, such as strong wind, cold, and drought. However, lignin has significant impact on the improvement of stress resistance. To explore the lignin biosynthesis process of A. carambola, the issue microstructure was visualized using paraffin sections, and the changes of lignin content and enzyme activity were measured at different development stages of twigs. Bioinformatic analyses based on genome sequencing and transcriptome profiling were conducted to identify structural genes and transcription factors that associated to the lignin biosynthesis. The results showed that the increase on the activity of three enzymes (PAL, CAD, and POD) and on the lignin content, as well as the appearance of xylem cells were observed along with the twigs development. A total of 11 structural genes, transcription factors (12 AcaMYBs and 11 AcaNACs) were identified in the A. carambola genome, followed by analyzing the differential expression patterns of these genes. By RT-qPCR, these genes AcaPAL1, AcaC3H2, AcaC4H2, AcaF5H1 and AcaMYB18, which exhibit obvious tissue specific expression pattern, might serve as key genes in tissue-specific lignin biosynthesis. Co-expression network analysis showed that AcaNAC29, AcaNAC39 and AcaMYB47 were the essential regulatory genes of lignin biosynthesis. Collectively, this study provided important information to facilitate future research on the transcription factors and structural genes of lignin biosynthesis in A. carambola.
Key words:  Averrhoa carambola L.  lignin  structural genes  transcription factors  expression pattern analysis