High temperature seriously threatens the yield, quality and fertility of middle rice varieties in Jianghan Plain. In order to identify rice varieties with high-temperature tolerance, this study analyzed the heat resistance at heading and flowering stages regarding new rice varieties in Hubei Province in the past three years, under natural high temperature environment (daily average temperature above 32 °C, the highest temperature above 35 °C and the duration above 5 days) and high temperature stress in glass greenhouse. Out of the varieties showing strong high-temperature resistance, the proportion of conventional varieties (lines) was equivalent to that of two-line hybrid rice combinations, and the proportion of three-line hybrid rice combinations was the second. A total of 20 varieties with strong heat resistance and better heat resistance were screened out in 3 year, among which 5 conventional varieties (lines) were Yuehesimiao, Runzhuxiangzhan, S532, Chunuo 3 and Lifengzhan. They can be used as heat-resistant germplasm resources for breeding and utilization, and can also be used as heat-resistant varieties after priority approval in the heat-damaged common areas. Quanyou 133, Quanyou Jingzhan and Quanyou Jinhe are three-line hybrid rice varieties, Cliangyou 300, Quanliangyou 5 and Enliangyou 454 are two-line hybrid rice varieties, which can be popularized and applied as heat-resistant varieties in heat-damaged common rice areas after the approval of the same conditions. The germplasm resources with excellent heat resistance may be screened out by further identification of their parents or one of their parents. A total of 102 heat-resistant varieties were identified and screened. The materials can be selected and approved according to the comprehensive performance of other traits, and then used as heat-resistant varieties in common rice areas. Among them, Enuo98, Hongnuo 1 and Longwangnuo 81 were conventional varieties ( lines ). Although their heat resistance is not too strong, most of them have excellent comprehensive agronomic traits, which can be used in the case of lack of heat-resistant breeding germplasm. 32 sub-varieties, such as Teyou 559, Huazheyou 1534 and Zhongguangyou 2, were sensitive upon heat treatment. It is suggested that priority should be eliminated at the time of approval, and special caution should be taken even in the promotion and application of approval.