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簇毛麦3V染色体在不同小麦遗传背景下的传递
张洁1, 蒋云1, 郭元林1, 王颖1, 杨燕2, 李小燕2, 邓自圆1, 邓光兵3, 宣朴4, 龙海3
0
(1.四川省农业科学院生物技术核技术研究所;2.四川师范大学;3.中国科学院成都生物研究所;4.四川省农业科学院农产品加工研究所)
摘要:
本研究前期创制了一份高抗小麦条锈病的新型中国春(CS)-簇毛麦3V(3D)代换系,CD-3,并将该条锈抗性初步定位于簇毛麦3V染色体上。为进一步探索簇毛麦3V染色体在不同小麦背景中的传递特性,对其育种利用提供依据,本研究将CD-3与地方小麦品种开县罗汉麦(KL)和推广小麦品种川麦42(CM42)分别进行正反交,F1代杂种自交后获得F2代群体。随后使用荧光原位杂交技术(FISH)研究了F2代群体3V的传递行为并调查了F2代植株的条锈抗性。结果显示:(1)四个组合中(CD-3/KL、KL/CD-3、CD-3/CM42和CM42/CD-3)F2代3V传递率为20.45%-34.94%,都极显著低于理论值(75%),组合之间无显著性差异,表明3V传递率在CM42和KL背景中传递率较低,且二者遗传背景对其传递率无影响。(2)组合CD-3/CM42 3V变异频率最高,与组合CD-3/KL和CM42/CD-3存在显著性差异,表明遗传背景对染色体结构稳定性有一定影响。(3)四个组合中,携带2条3V的植株均有较好的条锈抗性,携带1条3V的植株则表现感病或中抗,与亲本CM42或KL抗性一致,表明3V上的条锈抗性基因能在CM42和KL两种不同的遗传背景中能正常表达。本研究为深入研究该条锈抗性基因提供了理论依据,为小麦改良提供了材料基础。
关键词:  簇毛麦  3V染色体  传递率  染色体结构变异  条锈抗性
DOI:10.13430/j.cnki.jpgr. 20210427003
投稿时间:2021-04-27修订日期:2021-04-27
基金项目:国家自然科学基金(31801362);四川省科技厅应用基础项目(重点)(2019YJ0605, 2018JY0629);四川省财政厅创新能力提升工程(2019KXJJ-001);四川省农业科学院优秀论文基金(2018LWJJ-017);四川省农业科学院人才基金(2019LJRC020)
Transmission of 3V Chromosome from Dasypyrum villosum in Different Genetic Backgrounds of Common Wheat
ZANG Jie1, JIANG Yun1, GUO Yuan-lin1, WANG Ying1, YANG Yan2, LI Xiao-yan2, DENG Zi-yuan1, DENG Guang-bing3, XUAN Pu4, LONG Hai3
(1.Institute of Biotechnology and Nuclear Technology Research, Sichuan Academy of Agricultural Sciences;2.Sichuan Normal University;3.Chengdu Institute of Biology, Chinese Academy of Sciences;4.Institute of Agro-products Processing Science and Technology, Sichuan Academy of Agricultural Sciences)
Abstract:
In our previous study, CD-3, a novel CS-Dasypyrum villosum 3V (3D) substitution line with high resistance to wheat stripe rust, had been developed, and the stripe rust resistance had been mapped on the 3V chromosome. To explored the transmission character of the 3V chromosome and provide the theoretical basis for the utilization of stripe rust resistance from 3V in wheat breeding, CD-3 was directly and reciprocally crossed with landrace Kaixian Luohan Mai wheat (KL) and commercial variety Chuan Mai 42 wheat (CM42), respectively, in this study. The F1 hybrids were then selfed to obtain F2 plants for estimation of the transmission characters of the 3V chromosome using fluorescent in situ hybridization (FISH). At last, the stripe rust resistance of the F2 plants was investigated. The results showed: (1) The transmission rates of 3V in the 4 crosses (CD-3/KL, KL/CD-3, CD-3/CM42 and CM42/CD-3) ranged from 20.45% to 34.94%, which were significantly lower than the theoretical value (75%), and there was no significant difference among the 4 crosses, indicating that the genetic background had no influence on the transmission of 3V in this study; (2) The chromosomal structural aberration rate of 3V in CD-3/CM42 was significantly higher than that in CD-3/KL and CM42/CD-3, suggesting that the genetic background had influence on the chromosomal structural aberration rate of 3V; (3) The plants with two 3V chromosomes showed high resistance, and the plants carrying one 3V chromosome were susceptible or moderately resistant to stripe rust, which was consistent with the infection type of their parents, CM42 and KL. The result showed that the resistant gene(s) of stripe rust located on the 3V chromosome could express in the genetic background of the landrace KL and the commercial variety CM42. This research provided theoretical foundation for further exploration of the resistant gene(s) of stripe rust originating from the 3V chromosome of D. villosum, as well as valuable genetic materials for wheat improvement.
Key words:  Dasypyrum villosum  3V chromosome  transmission rate  chromosomal structural aberration  resistance to stripe rust