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抗稻瘟病基因Pi2的基因特异性KASP标记开发与应用
杨义强1, 朱林峰1, 李晓芳1, 付 杰1, 黄道强1, 邱先进2, 周少川1, 王重荣1
0
(1.广东省农业科学院水稻研究所/广东省水稻新技术研究重点实验室;2.长江大学)
摘要:
水稻稻瘟病抗性基因Pi2是一个对稻瘟病生理小种具有广谱抗性的基因,对于水稻的稻瘟病抗性育种具有非常重要的应用价值。为提高Pi2的选择效率,本研究根据高抗稻瘟病品种黄广油占与高感稻瘟病品种广陆矮4号在Pi2基因的第787位、第788位密码子上的变异GCA GGA/GTG TTA,基于竞争性等位基因PCR(kompetitive allele specific PCR,KASP)标记技术原理,开发Pi2的基因特异性KASP标记。对40个水稻品种的检测结果表明,所开发的基因特异性KASP标记可准确区分Pi2基因的抗性等位基因型、感病等位基因型和杂合基因型,并且与抗病表型具有高度关联,是一种鉴定Pi2基因的抗性等位基因的有效方法。在F2分离群体的应用结果表明,该标记可在苗期检测育种材料的稻瘟病抗性基因Pi2的等位基因型,不需要将育种材料种到病圃鉴定,可用于预测育种材料的稻瘟病抗性,提高育种效率。本研究鉴定出了27个携带Pi2抗稻瘟病基因的广东抗病水稻品种,并提供了可快速鉴定的分子标记,对提高品种培育效率,促进抗稻瘟病水稻品种的遗传改良,具有重要应用价值。
关键词:  水稻  抗稻瘟病基因  基因特异性标记  KAPS标记  MAS
DOI:10.13430/j.cnki.jpgr. 20210327001
投稿时间:2021-03-27修订日期:2021-05-06
基金项目:广东省农业科学院农业优势产业学科团队建设项目(202111TD);广东省应用型科技研发专项(2015B020231001)
Development and Application of KASP Marker Specific for Rice Blast Resistance Pi2 Gene
YANG Yi-qiang1, ZHU Lin-feng1, LI Xiao-fang1, FU Jie1, HUANG Dao-qiang1, QIU Xian-jin2, ZHOU Shao-chuan1, WANG Chong-rong1
(1.Rice Research Institute, Guangdong Academy of Agricultural Sciences / Guangdong Key Laboratory for New Technology Research of Rice;2.Yangtze University, Hubei Jingzhou)
Abstract:
Pi2 is a rice gene for broad-spectrum resistance to blast pathogens, which has an important application value for rice blast resistance breeding in South China. In order to improve the selection efficiency, a KASP marker specific for Pi2 gene was developed based on the variation GCA GGA / GTG TTA at codon 787 and 788 of Pi2 gene between the highly resistant variety Huangguang Youzhan and the highly susceptible variety Guanglu Ai 4. The developed allele specific marker could accurately distinguish the resistance allele, susceptible allele and heterozygous genotype at Pi2 in 40 rice varieties, which was highly correlated with their resistance/susceptible phenotypes. Further more, this marker was applied in a large F2 segregation population from a cross of Wushan Youzhan and Yujing 91 to select the target genotypes of the individuals or lines that carried the blast resistance Pi2 alleles through marker-assisted selection exhibited intermediate or high resistance to blast in disease nursery. In conclusion, using the developed allele specific marker, 27 Guangdong rice varieties that carry blast resistance gene Pi2 were identified, which has an important application value in improving selection efficiency in the development of blast resistant rice varieties.
Key words:  rice  Blast resistance gene  Allele specific marker  KASP marker  MAS