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江西大禾谷资源的籼粳分化与品质分析
李 霞1, 花旺忠2, 王雪松1, 唐书升1, 吴 婷1, 罗 鑫1, 肖宇龙1, 王智权1, 余传源1, 余丽琴1, 王晓玲1
0
(1.江西省农业科学院水稻研究所;2.弋阳县水稻原种场)
摘要:
江西大禾谷资源丰富,品质特异,深受欢迎,但遗传研究与品质改良均未见报道,株型急需改良。本研究利用SNP标记对弋阳大禾谷进行遗传分析,RVA进行品质测定。结果显示,40对SNP引物对20份材料检测出82个等位基因,其中大禾谷具有籼型基因等位频率在0.025~0.100之间,属粳稻类型。大禾谷的RVA黏度性状是稻米成熟的一个积累过程,而AC(amylose content)含量则在稻米成熟前就已定型。RVA参数与一些理化性状密切关联,尤其是米粒长可以负反应崩解值;整精米率和AC含量是主基因性状;与偏软型栽培稻相比,峰值时间是判断大禾谷的一个关键参数。通过SNP和RVA分析,选出了品质接近、性状互补的优良粳稻亲本,如农局Ⅲ号(麻壳)最软,麻壳棉花大品质最稳定,农科所白壳大是育种中的优选长粒资源,皖运粳11036是大禾谷株型改良的优选材料,这些结果将为大禾谷的遗传改良指明了方向。
关键词:  大禾谷  SNP  籼粳分化  品质
DOI:10.13430/j.cnki.jpgr. 20210218003
投稿时间:2021-02-18修订日期:2021-05-06
基金项目:第三次全国农作物种质资源普查与收集行动;江西省青年基金重点联合项目(20192ACBL21024);国家自然科学基金(32060475);海南省重大科技计划项目(ZDKJ202002)
Indica-Japonica Differentiation and Quality Analysis of Dahegu Rice Resources in Jiangxi Province
LI Xia1, HUA Wang-zhong2, TANG Shu-sheng1, WU Ting1, LUO Xin1, XIAO Yu-long1, WANG Zhi-quan1, YU Chuan-yuan1, YU Li-qin1, WANG Xiao-ling1
(1.Rice Research Institute, Jiangxi Academy of Agricultural Sciences;2.Yiyang County Rice Original Breeding Farm, Jiangxi Yiyang)
Abstract:
Dahegu rice resources in Jiangxi Province are rich and are favorable for their special quality. No genetic study and quality improvement of Dahegu rice have been reported, and the plant stature is in need of improvement. In this study, SNP (single nucleotide polymorphism) markers were used to analyze the genetic characteristics of Dahegu rice varieties from Yiyang, and RVA (Rapid Visco Analyser) was used for quality determination. The results showed that 40 pairs of SNP primers detected 82 alleles in 20 materials, among which Dahegu rice varieties had indica frequency between 0.025 and 0.100, and were determined to be of japonica rice type. The viscosity of Dahegu rice grain was an accumulation process through grain maturation, whereas the amylose content was fixed before grain maturation. RVA parameters were closely related to physicochemical properties, and especially the rice grain length was negatively correlated with breakdown value. Pure milled rice grain rate and amylose content were the major gene traits. Compared with soft cultivated rice, the peak time was a key parameter to judge Dahegu rice. Through the screening analysis of the SNP and the viscosity, excellent japonica parents with similar quality and complementary traits were selected, such as brown-chaffed Nongju Ⅲ with the softest grain texture, brown-chaffed Mianhuada with the most stable quality, long-grained Nongkesuo Baikeda as a favorable breeding resource, and Wanyunjing 11036 as a choice breeding material for improving plant stature of Dahegu rice. These results would provide a guidance for the genetic improvement of Dahegu rice.
Key words:  Dahegu rice  single nucleotide polymorphism  indica-japonica differentiation  rice quality