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糜子矮秆突变体m819表型性状及其矮化机制研究
朱学海1, 贾小平2, 魏玮1, 刘洋1, 张帅1, 郭英杰1, 武永祯1, 王瑶1, 寇淑君1
0
(1.张家口市农业科学院;2.河南科技大学农学院)
摘要:
创制糜子矮秆种质,开展糜子矮化育种,具有重要的理论意义和应用价值。利用 EMS (甲基磺酸乙酯)诱变石湖千斤糜(种植序号260),获得一个稳定遗传的糜子矮杆突变体819,本研究从形态学、细胞学、分子生物学的角度对其农艺性状、生理特性及矮化机制进行研究。结果发现:该突变体苗期即表现矮化,一直保持到成熟,株高仅57.1cm。与其野生型相比,突变体的各茎节均极显著地缩短,茎节数差异不显著,突变体分蘖数显著增加达到极显著水平,穗柄长、穗长、千粒重下降达到极显著水平,茎粗、叶长无明显变化,叶宽变宽达到极显著水平,突变体叶色深绿,抽穗期剑叶、倒二叶、成熟期剑叶叶绿素含量均高于野生型,其中抽穗期倒二叶叶绿素含量与野生型差异达到极显著水平。通过主茎剖面细胞学观察发现,造成矮化的主要原因是茎节纵向细胞数目减少。突变体与高秆材料J12构建的F2分离群体株高频次呈单峰分布,说明矮秆突变体819属于多基因控制的数量性状。突变体成熟期植株叶片内源 GA3+1含量显著高于野生型,突变体对外源噴施GA3反应不敏感,说明本突变体属于GA信号传导缺陷型矮秆突变体。利用BSA-Seq法鉴定矮杆候选基因,发现一个赤霉酸不敏感基因(登录号:PM01G30960),推测该基因是矮杆突变体819形成的关键基因,这为糜子矮杆新品种的开发及应用奠定了基础。
关键词:  糜子  矮秆突变体  农艺性状  GA3  矮杆基因
DOI:10.13430/j.cnki.jpgr.20210213001
投稿时间:2021-02-13修订日期:2021-03-16
基金项目:张家口市科技局项目(1911016C-3);河北省高层次人才资助项目(A201903020);国家产业技术体系子项目(CARS-06013.5-B6-1)
Deciphering the Phenotypic Variation and Dwarfing Mechanism of a Dwarf Mutant m819 in Panicum miliaceuml L.
ZHU Xue-hai1, JIA Xiao-ping2, WEI Wei1, LIU Yang1, ZHANG Shuai1, GUO Ying-jie1, WU Yong-zhen1, WANG Yao1, KOU Shu-jun1
(1.Zhangjiakou Academy of Agricultural Sciences;2.College of Agronomy,Henan University of Science and Technology)
Abstract:
Creating dwarfmutant germplasm in Panicum miliaceuml and developing Panicum miliaceuml breeding isof great theoretical significance and valuable on application. A character-stably-heritable dwarfmutant in Panicum miliaceuml was induced from Shi hu qian jin mi (planting number 260) by EMS (ethyl methyl sulfonic acid) mutagenesis. The mutants’ agronomic traits, physiological characteristics and dwarfing mechanism were studied from the perspective of morphology, cytology and molecular biology. The results showed that :The mutant was dwarfed at the seedling stage and it would maintain until maturity, the plant height was only 57.1cm. Compared with the wild type,the mutantinternodes were extremely significantly shortened, internodes number has insignificantdifference, tiller number increased significantlyreaching extremely significant level, the drop of pedicel length, ear length and thousand-grain weight reached extremely significant level, no obvious changes appeared on the stem diameter and leaf length, the increase of leaf widthreached extremely significant level and the mutant leaf greened darkly. The chlorophyll contents in sword leaf and top second leaf at heading stage and the sword leaf at mature stage were higher than these in wild type, of whichthe chlorophyll content difference in top second leaf reached significant level compared with wild type. According to the cytological observation of the main stem profile, it was found that the main cause of dwarfing was the decrease of the number of longitudinal cells in the stem node. The plant height frequency of F2 segregation population constructed by the mutant and the high straw J12 distributed unimodally, indicating that the dwarf mutant 819 belonged to quantitative trait inheritance controlled by multiple genes. The endogenous content of GA3+1 in mutant leaves at maturity stage was significantly higher compared with wild type, and the mutant was insensitive to exogenous GA3, indicating thatthe mutant was a GA signal transduction defect type dwarf mutant. A gibberellic acid insensitive gene (accession number: PM01G30960) was identified by BSA-Seq method, which was assumed to be the key gene for the formation of dwarf mutant 819, which laid a foundation for the development and application of new dwarf varieties in Panicum miliaceuml.
Key words:  Panicum miliaceuml  dwarf mutant  agronomic traits  GA3  dwarf gene