Citation:[Copy]
[Copy]
【Print page】 【Online reading】【PDF Full text】 查看/发表评论下载PDF阅读器关闭

←Previous page|Page Next →

Back Issue    Advanced search

This Paper:Browse 476   Download 525 本文二维码信息
码上扫一扫!
小麦种质抗吸浆虫鉴定及抗虫 SSR 标记开发
章悦1, 王伊瑾2, 梁虹1, 张力菁1, 耿妙苗1, 刘桂茹1, 王睿辉1
0
(1.河北农业大学农学院/华北作物调控与改良国家重点实验室;2.河北农业大学信息科学与技术学院)
摘要:
麦红吸浆虫(Sitodiplosis mosellana Géhin)是严重威胁小麦生产的重要害虫,其生活习性隐蔽,防治困难,筛选抗虫 小麦种质及可用于抗虫性辅助选择的分子标记意义重大。本研究对我国不同麦区、不同时期的 566 份小麦种质资源进行了吸 浆虫抗性鉴定,并尝试从抗虫性主效 QTL 所在基因组区段开发新的 SSR 标记。结果表明,27.03% 的供试小麦种质对吸浆虫 高抗或免疫,那些早期选育的抗虫品种依然具有很好的吸浆虫抗性。同时,从前期定位的抗麦红吸浆虫主效位点 Qsm.hbau- 4A 所在区段中开发出 565 个 SSR 标记,并以 11 个具有较好多态性的 SSR 标记,在 566 份小麦种质资源中对这些标记的检测 效率进行了验证,其中标记 Xhs87201bp 的扩增效果好、检测效率较高(平均 65.37%),可以用于抗虫种质资源筛选和分子标记辅 助选择。
关键词:  小麦  麦红吸浆虫  SSR 标记  抗虫种质资源  标记开发
DOI:10.13430/j.cnki.jpgr.20210211003
投稿时间:2021-02-11修订日期:2021-04-02
基金项目:国家自然科学基金(32072000);河北省自然科学基金(C2020204034)
OWBM-resistance Screening for Wheat Germplasm and SSR Marker Development in Major Resistant Qsm.hbau-4A
Zhang Yue1, Wang Yi-jin2, Liang Hong1, Zhang Li-jing1, Geng Miao-miao1, Liu Gui-ru1, Wang Rui-hui1
(1.College of Agronomy, Hebei Agricultural University/Key laboratory of Research and Utilisation of Crop Germplasm Resaurces in North China Ministry of Education;2.College of Information Science & Technology, Hebei Agricultural University)
Abstract:
Orange wheat blossom midge(OWBM,Sitodiplosis mosellana Géhin) is an important pest insect that seriously threatens wheat production. Cryptic habitat of OWBM lifecycle made pest control very difficult. Therefore,screening for OWBM-resistant wheat germplasm and tightly-linked molecular marker is of great important for pest control. In this study,566 wheat germplasm resources from different wheat regions and different periods,and genomic regions surrounding major OWBM locus were used to identify their resistance to OWBM,and to discover new SSR markers,respectively. The results showed that 27.03% of wheat cultivars were immune or highly resistant to OWBM,of which released in early years were still resistant to OWBM. Meanwhile,565 SSR markers were developed from the genomic region of major OWBM-resistant locus(Qsm. hbau-4A),and their detection efficiency were tested among this panel of wheat accessions. Of these SSR markers, marker Xhs87201bp showed robust PCR amplification and presented an average of 65.37% detection efficiency,and can be used for marker-assisted selection of OWBM resistance.
Key words:  bread wheat(Triticum aestivum L.)  orange wheat blossom midge(Sitodiplosis mosellana Géhin)  SSR markers  OWBM-resistant germplasm resources  marker development