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小麦芒基因定位及其与农艺性状的相关性分析
李玲, 刘盼, 张蕾, 张浩, 贾继增, 高丽锋
0
(中国农业科学院作物科学研究所,北京100081)
摘要:
芒是位于植物穗上的针状结构,广泛存在于禾本科作物水稻、小麦、高粱和大麦中,不同作物芒的结构存在差异。 小麦中,芒对提高穗光合效率和产量、防鸟、抗虫及抗逆有重要作用。前人已经对抑制小麦芒发育的主要基因进行了定位和 遗传分析,四个主效基因中仅有B1(Tipped1)基因被克隆。本研究基于人工群体云南3 号和偃展1 号BC3F6 群体(YN3/YZ1) 和自然群体,分析了芒与其他农艺性状的关系,发现芒对株高和产量性状有显著影响;用小麦660K SNP 芯片扫描YN3/YZ1 和自然群体,全基因组关联分析(GWAS)显示小麦染色体5AL 和6BL 存在与芒性状显著相关的基因组区域,分别对应于小 麦芒抑制基因B1 和B2;长芒和顶芒近等基因系转录组分析发现,在6BL 候选区间内有23 个差异表达基因。本研究将为进一 步克隆B2 基因提供参考。
关键词:  小麦    小麦660K SNP芯片  GWAS  RNA-seq
DOI:10.13430/j.cnki.jpgr.20200505001
投稿时间:2020-05-05修订日期:2020-05-29
基金项目:国家自然科学基金项目(31971882)
Awn Genes Mapping and Correlation Analysis for Agronomic Traits in Wheat
LI Ling, LIU Pan, ZHANG Lei, ZHANG Hao, JIA Ji-zeng, GAO Li-feng
(Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081)
Abstract:
Awn is a needle-like structure located on the ear of plants. It is widely found in the grass family such as rice, wheat, sorghum and barley, and the structure of awn varies in different crops. In wheat, awn plays an important role in improving the photosynthetic efficiency and yield, and in preventing damages caused by pest birds, insects and adverse conditions. Genes related to the development of wheat awn have already been mapped and genetically analyzed, but only one of the four major awn-inhibiting genes, B1 (Tipped1), has been cloned. In this study, conducted on a BC3F6 genetic population (YN3/YZ1) and a natural population, we estimated the relationship between the awn and agronomic traits, and found that awn had significant effects on plant height and yield. Genome-wide association studies (GWAS) based on wheat 660K SNP array identified genomic regions on chromosomes 5AL and 6BL significantly related to awn trait, which corresponded to awn-inhibiting genes B1 and B2 respectively. From transcriptome data of long-awned and tip-awned near-isogenic lines (NILs), 23 differentially expressed genes were identified from the 6BL candidate interval. These results will facilitate the B2 gene cloning in the future.
Key words:  wheat  awn  Wheat 660K SNP array  GWAS  RNA-seq

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