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四倍体大白菜雄性不育系资源的创制
刘晓东, 王明秋, 孟川, 吴芳, 马蕾, 牟金贵, 王玉海
0
(河北省农林科学院经济作物研究所/河北省蔬菜工程技术中心,石家庄 050051)
摘要:
目前国内外对二倍体大白菜雄性不育系的创制已进行了深入的研究,利用雄性不育系技术育种已相对成熟,而四 倍体大白菜育种仍以突破稔性、提高结籽率的自交系选育为主,四倍体雄性不育的研究尚无相关报道。本研究通过从二倍体 大白菜细胞质雄性不育系中筛选合适的含新型甘蓝型油菜 CMS 基因的资源(CMS 后 36 高)为不育源,依靠人工秋水仙素加倍, 经倍性鉴定和高代稳定四倍体自交系 D571、D574 连续多代回交转育,成功获得了 CMS-D571、CMS-D574 两个四倍体大白菜细 胞质雄性不育系,该不育系具有不育度达 100%、结籽率高、遗传稳定的特点。该研究证实细胞质雄性不育性也可在四倍体大 白菜育种上应用,可以作为创制四倍体大白菜细胞质雄性不育系的方法,为四倍体大白菜开展雄性不育系育种奠定了物质基础。
关键词:  四倍体  大白菜  雄性不育系  创制
DOI:10.13430/j.cnki.jpgr.20200415004
投稿时间:2020-04-15修订日期:2020-04-28
基金项目:国家重点研发计划(2016YFD0101701);河北省科学技术研究与发展计划(16226304D-1);国家大宗蔬菜产业技术体系项目(CARS-23-G-05)
Innovation of Male Sterile Lines in Tetraploid Chinese Cabbage
LIU Xiao-dong, WANG Ming-qiu, MENG Chuan, WU Fang, MA Lei, MU Jin-gui, WANG Yu-hai
(Institute of Cash Crops of Hebei Academy of Agricultural and Forestry Sciences/Hebei vegetable Engineering Technology Centre,Shijiazhuang 050051)
Abstract:
The male sterile lines of diploid Chinese cabbage have been widely investigated for theoretical studies and practical uses. In tetraploid Chinese cabbage, improvement of fertility and seed setting rate in self-bred lines was favorable, while study of the male sterile lines remained un-investigated. In this study, a sterile source (CMS hou36gao) with new Brassica napus cytoplasmic male sterile (CMS) gene was selected from the diploid Chinese cabbage, and subjected for chromosome doubling using colchicine treatment. By multiple consecutive cycles with tetraploid inbred lines D571 and D574, in conjugation with cytogenetically identifying the number of chromosomes, two tetraploid lines CMS-D571 and CMS-D574 that showed cytoplasmic male sterile were produced. Both lines showed sterility rate up to 100% with high seed setting rate and genetic stability. This study raised an example by using the cytoplasmic male sterility in tetraploid Chinese cabbage breeding, and established an experimental pipeline to generate the cytoplasmic tetraploid male sterile lines. Collectively, this work laid a foundation for innovation and practical use of male sterile lines in tetraploid Chinese cabbage.
Key words:  tetraploid  Chinese cabbage  male sterile line  innovation

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