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基于诊断标记的无LOX-1活性大麦种质鉴定评价
赵海鹏1, 赵雪芳2, 孙建1, 窦婷语2, 张仁旭2, 阿卜来提.阿力木2, 郭爱奎2, 王春超2, 王化俊1, 张京2, 孟亚雄1, 郭刚刚1
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(1.甘肃农业大学农学院/甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室;2.中国农业科学院作物科学研究所)
摘要:
在啤酒酿造和储藏过程中,所含脂类物质代谢是影响啤酒风味稳定性和啤酒货架期长短的主要原因。研究表明, 大麦脂肪氧化酶 I(LOX-1)是导致脂类代谢的关键酶,筛选和创制 LOX- 1 活性缺失(null LOX-1)的大麦种质是促进啤酒 大麦品质育种的有效途径。为提高检测效率,针对前期鉴定出的 null LOX-1 中国大麦稀有 SNP 变异,开发出特异性快速诊 断标记,并利用该标记对来源于河南省和山东省共 633 份大麦地方品种进行鉴定评价,共计筛选出 8 份新种质,同时明确了 该变异的地理分布及其在不同地方品种中的变异频率。本研究不仅为啤酒大麦分子辅助育种提供了优异种质和快速检测手 段,也为大麦种质资源遗传完整性保护与利用提供了参考。
关键词:  大麦  种质资源  遗传完整性  鉴定评价
DOI:10.13430/j.cnki.jpgr.20200405001
投稿时间:2020-04-05修订日期:2020-04-25
基金项目:国家重点研发计划项目(2018YFD1000706-1);小宗作物种质资源保护与利用专项(2019NWB036-06);国家大麦青稞产业技术体系建 设专项(CARS-05)
Identification of null LOX-1 Barley Germplasm based on a Newly-developed Diagnostic KASP Marker
ZHAO Hai-peng1, ZHAO Xue-fang2, SUN Jian1, DOU Ting-yu2, ZHANG Ren-xu2, Ablat Alim2, GUO Ai-kui2, WANG Chun-chao2, WANG Hua-jun1, ZHANG Jing2, MENG Ya-xiong1, GUO Gang-gang1
(1.College of Agronomy, Gansu Agricultural University/ Key Laboratory of Arid Land Crop Science in Gansu Provice / Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement;2.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences)
Abstract:
The components, which are derived from the lipid metabolism of barley malt, destabilizes the beer flavor and its shelf life in the brewing industry. The lipoxygenase 1 (LOX-1) encoded by the LOX-1 gene has been approved as the key enzyme involved in lipid metabolism. The identification of barley germplasm without a functional LOX-1 gene might be of interest to promote high-quality malting barley breeding. By taking use of the previously-identified null LOX-1 Chinese barley rare SNP, here a Kompetitive allele specific PCR (KASP) based diagnostic marker was generated and subjected for a test in 633 barley landraces which originated from Shandong and Henan Province. A total of eight accessions harboring a loss-of-functional LOX-1 allele were identified, and the geographic distribution and the allele frequency were further investigated. Taken together, this work provided several LOX-1 free germplasm accessions valuable for the malt barley breeding especially in conjugation with the newly-generated KASP diagnostic marker. Moreover, this study is an example that highlights the importance of the barley genetic integrity in protection and utilization of germplasm resources.
Key words:  barley  germplasm resources  genetic integrity  identification and evaluation