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大豆种质资源萌发期耐旱性评价
张海平1, 张俊峰1, 陈妍1, 张海生2, 闫凯1
0
(1山西农业大学农业基因资源研究中心(山西省农业科学院农作物品种资源研究所)/农业部黄土高原作物基因资源与种质创制重点实验室/杂粮 种质资源发掘与遗传改良山西省重点实验室,太原030031;2山西省农业科学院作物科学研究所,太原030031)
摘要:
随着全球气候的变化,干旱已成为限制大豆产量的重要环境因素之一,大豆种质资源耐旱性鉴定对大豆耐旱品种 的培育及大豆耐旱机理的研究具有重要的意义。本研究首先选择4 份耐旱性不同的材料,以PEG-6000 作为渗透胁迫剂,研 究了大豆种质在0%、10%、15%、16%、18%、20%、30%的PEG-6000 溶液的胁迫下,相对发芽率、相对发芽势、相对胚根 长、萌发耐旱指数的变化。结果表明15% ~ 20% PEG-6000 溶液是大豆萌发期耐旱鉴定的适宜浓度范围。本研究采用16% PEG-6000 溶液作为胁迫浓度,对568 份大豆资源进行大豆种子萌发期耐旱鉴定,以相对发芽势、相对发芽率、相对胚根长、 相对苗高、萌发耐旱指数、活力指数作为评价指标,应用平均隶属函数评价法对大豆种质萌发期耐旱性进行综合评价,筛选 出耐旱种质4 份,较耐旱种质18 份,中间型种质110 份,较敏感种质194 份,敏感型种质242 份。
关键词:  大豆种质  萌发期  耐旱鉴定  隶属函数评价
DOI:10.13430/j.cnki.jpgr.20200330001
投稿时间:2020-03-30修订日期:2020-07-03
基金项目:“十三五”重点研发计划课题(2016YFD0100201);山西省农业科学院农业科技创新研究课题(YCX2018401);山西省重点研发计划项目 (201903D211003)
Identification and Evaluation of Soybean Germplasm Resources for Drought Tolerance During Germination Stage
ZHANG Hai-ping1, ZHANG Jun-feng1, CHEN Yan1, ZHANG Hai-sheng2, YAN Kai1
(1Center for Agricultural Genetic Resources Research, Shanxi Agricultural University(Institute of Crop Germplasm Resources,Shanxi Academy of Agricultural Sciences)/ Institute of Crop Germplasm Resources of Shanxi Academy of Agricultural Sciences / Key Laboratory of Genetic Resources and Genetic Improvement, Tai Yuan 030031; 2Institute of Crop Sciences, Shanxi Academy of Agricultural Sciences, Tai Yuan 030031)
Abstract:
With the change of the global climate, the drought tolerance at germination stage of soybean becomes of importance when suffering spring drought. In order to explore an optimal drought stress condition, four soybean accessions with levels of drought tolerance were tested with the concentrations of 0%, 10%, 15%, 16%, 18%, 20%, 30% of PEG-6000 solution simulated different drought conditions. The result showed that 15% ~ 20% of PEG-6000 solution was qualified to simulate drought stress condition at germination stage of soybean. Moreover, 16% of PEG-6000 solution was applied to culture the seeds of 568 soybean germplasm accessions. The indexes were estimated using six indicators including relative germination energy, relative germination rate, relative radical length, relative bud length, germination drought tolerant index and vigor index. Based on subordination function method and the standard classification of drought tolerance in soybean, four drought tolerant varieties, 18 medium tolerant varieties, 110 intermediate type varieties, 194 relatively drought sensitive varieties and 242 drought sensitive varieties were identified.
Key words:  soybean  germination stage  drought resistance  subordination function method

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