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镉低积累水稻资源的镉积累稳定性及遗传相似性分析
赵文锦1, 黎用朝1,2,3, 李小湘1,2,3, 潘孝武1,2,3, 刘文强1,2,3, 熊海波2,3, 董铮2,3, 魏秀彩1, 刘利成2,3, 刘三雄2,3, 闵军2,3, 段永红2,3, 余亚莹2,3, 盛新年2,3
0
(1湖南大学研究生院隆平分院,长沙410125;2湖南省农业科学院水稻研究所,长沙410125;3农业部长江中下游籼稻遗传育种重点实验室,长沙410125)
摘要:
本研究以9311为高镉对照,湘晚籼12号为相邻低镉对照,2015年~2017年经田间鉴定筛选出的15份镉低积累水稻资源为参试材料,鉴定精米的相对降镉率并筛选出年度间相对降率差异不显著的资源;采用45对SSR引物检测15份资源的遗传多样性,提出遗传距离大且相对降镉率高的育种可用资源。2018年试验结果表明,15份低镉水稻资源的精米镉含量都低于湘晚籼12号,相对降镉率大于50%的有10份,变幅为50.8%~83.5%;小于50%的有5份,变幅是1.9%~49.8%;2019年试验结果表明,7份生育期相近的资源,相对降镉率都大于50%,变幅是60.1%~78.7%;2年试验结果的综合分析表明,4份低镉资源7(BS82)、14(X211)、19(7W172)、20(7W216)的镉积累在年度间无显著性差异,且相对降镉率都在50%以上。15份镉低积累资源总体遗传多样性较为丰富(Na=4.311、Ne=3.257、Ho=0.041、He=0.657、I=1.207、Nei's=0.639、PIC=0.612);遗传相似系数(GS)的平均值为0.377,亲缘关系较远,材料7和材料14、材料7和材料19(20)、材料14和材料19(20)的遗传相似系数分别为0.319、0.447、0.426。由上说明BS82、X211、7W172、7W216是镉低积累新品种选育的可利用的宝贵资源。
关键词:  镉低积累  水稻  鉴定  遗传多样性
DOI:10.13430/j.cnki.jpgr.20191226001
投稿时间:2019-12-26修订日期:2020-05-21
基金项目:国家和湖南省财政专项镉低积累水稻品种选育;“十三五”国家重点研发项目(2016YFD0100101-12)
Stability of Cadmium Accumulation and Genetic Similarity Analysis in Low-accumulation Rice Resources
ZHAO Wen-jin1, LI Yong-chao1,2,3, LI Xiao-xiang1,2,3, PAN Xiao-wu1,2,3, LIU Wen-qiang1,2,3, XIONG Hai-bo2,3, Dong Zheng2,3, WEI Xiu-cai1, LIU Li-cheng2,3, LIU San-xiong2,3, MIN Jun2,3, DUAN Yong-hong2,3, YU Ya-ying2,3, SHENG Xin-nian2,3
(1Long Ping Branch,Graduate School of Hunan University,Changsha 410125;2Hunan Rice Research Institute, Hunan Academy of Agriculture Science, Changsha 410125;3Key Laboratory of Indica Rice Genetics and Breeding in the Middle and Lower Reaches of Yangtze River Valley, Ministry of Agriculture,P.R.China,Changsha 410125)
Abstract:
the relative Cd-reduction rate of polished rice and screened the resources with no significant difference in the annual relative Cd-reduction rate. In addition, 45 SSR markers were used to detect the genetic diversity of 15 rice accessions, in order to identify the resources available for breeding with a great genetic distance and high relative Cd-reduction rate. The results showed that in 2018, Cd content in 15 low-Cd polished rice was lower than cv. Xianwanxian 12. Ten samples represented relative Cd-reduction rate of more than 50%, ranging from 50.8% to 83.5%, while 5 samples were less than 50%, ranging from 1.9% to 49.8%. In 2019, the relative Cd-reduction rate in 7 rice samples with similar growth period was higher than 50%, variable from 60.1% to 78.7%. Based on the two-year test results, no significant difference was found among four low-Cd rice samples 7 (BS82), 14 (X211), 19 (7W172), 20 (7W216), and the relative Cd-reduction rate was higher than 50%. The overall genetic diversity in 15 low Cd-accumulating rice population indicated relatively abundant (Na=4.311, Ne=3.257, Ho=0.041, He=0.657, I=1.207, Nei's=0.639, PIC=0.612). The mean genetic similarity (GS) was 0.377, suggesting distant genetic relationship. For examples, the GS in pairs of samples 7 and 14, samples 7 and 19 (20) as well as samples 14 and 19 (20) were 0.319, 0.447 and 0.426, respectively. Therefore, four rice germplasm resources
Key words:  low Cd-accumulating  rice  identify  genetic diversity