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甜荞等花柱资源与栽培甜荞杂交初步研究
陈稳良1, 李秀莲1, 史兴海1, 梁改梅2, 刘龙龙3, 赵建栋3, 秦秀珍1
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(1.山西省农业科学院作物科学研究所;2.山西省农业科学院旱地农业研究中心;3.山西省农业科学院农作物品种资源研究所)
摘要:
探讨等花柱资源在甜荞资源创新中有效利用的方法,为今后甜荞资源创新及新品种选育提供更好的技术支撑。利用甜荞等花柱资源与栽培种不同花型进行正反交杂交试验,在此基础上对等花柱×栽培种、等花柱×长花柱、等花柱×短花柱、栽培种×等花柱、长花柱×等花柱、短花柱×等花柱6个杂交组合后代结籽状况及遗传分离进行观察和分析。结果表明,等花柱甜荞资源本身结实率达22.6%,明显高于普通甜荞,对于等花柱新纯系的筛选以“长花柱×等花柱”杂交模式最佳,新品系筛选是“等花柱×栽培种”最佳。本研究结果说明根据资源创新的目的不同,利用等花柱资源采用不同的杂交技术是可行的,后代都产生了明显的遗传变异,能有效解决甜荞杂交育种的瓶颈问题。
关键词:  甜荞  等花柱资源  杂交  资源创新
DOI:10.13430/j.cnki.jpgr.20191219002
投稿时间:2019-12-19修订日期:2020-05-21
基金项目:国家燕麦荞麦现代农业产业技术体系专项资金(CARS-07-A-2);山西省科技攻关项目(201903D221096);山西省科技成果转化项目 (201804D131053, 201904D131059);山西省科技成果转化项目;山西省农业科学院特色农业技术攻关项目(YGG17128);山西省农业科 学院育种工程项目(17yzgc092);山西省杂粮产业技术体系(sxzljstx-1)
A Preliminary Study on the Hybridization of Common Buckwheat Isostyle Resources and Cultivated Buckwheat
CHEN Wen-liang1, LI Xiu-lian1, SHI Xing-hai1, LIANG Gai-mei2, LIU Long-long3, ZHAO Jian-dong3, QIN Xiu-zhen1
(1.Institute of Crop Science, Shanxi Academy of Agricultural Sciences;2.Dry land Agriculture Research Center, Shanxi Academy of Agricultural Sciences;3.Institute of Crop Germplasm Resources,Shanxi Academy of Agricultural Sciences)
Abstract:
In order to provide better guarantee on technical support for the future innovation of common buckwheat resources and new variety breeding, this study investigated the effective method of isostyle resources in innovation of common buckwheat. The positive and negative hybridization tests by using isostyle germplasm resource crossing with common buckwheat cultivars showing different flower types were carried out. Six hybridization combinations (isostyle × cultivar, isostyle × long style, isostyle × short style,cultivar × isostyle, long style × isostyle, short style × isostyle) were achieved, and they were subjected for analyzing the seed-fertility and the inheritance segregation. The results showed that the fertility rate of isostyle common buckwheat resources was observed up to 22.6%, significantly higher than that of common buckwheat. Regarding to the hybrids for selecting the new isostyle pure lines, the "long style × isostyle" hybrid pattern was the best pattern. Otherwise, the "isostyle × cultivar" was optimal for the selection of new strains. Taken together, this study suggested the feasibility on hybridization techniques in order to meet the demand of the resource innovation. The offspring plants were observed with obvious phenotypic variation, which might suggest a possibility to effectively solve the bottleneck problem in common buckwheat cross breeding.
Key words:  common buckwheat  isostyle resource  hybridization  resource innovation