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定向改良超级早稻中早39稻瘟病抗性
曹妮, 季芝娟, 曾宇翔, 郑安福, 方晶璟, 杨长登, 梁燕
0
(中国水稻研究所)
摘要:
稻瘟病是水稻生产中最具毁灭性的病害之一,稻瘟病菌生理小种变异快,抗性品种推广 3-5 年后抗性衰退风险较 高,改良及培育抗稻瘟病菌新致病小种的品种是防治该病害最经济有效的方法。本研究以携带稻瘟病抗性基因 Pi25 的材料 R6 为供体亲本,超级早稻中早 39 为受体亲本和轮回亲本,通过杂交、回交和自交以及分子标记辅助选择技术,并结合室内 喷雾接种、田间注射接种叶瘟以及病圃自然发病区穗颈瘟鉴定。综合考量抗性表型、基因型以及农艺性状,筛选出 4 个携带 Pi25 基因,叶瘟及穗颈瘟抗性较中早 39 提高,且农艺性状良好的株系 FY82、FY90、FY125、FY137,为培育抗稻瘟病品种 提供中间材料。同时对育成品系的系谱组成和抗性相关位点进行研究,初步阐明了育成品系较轮回亲本抗性水平提高的遗传 基础。
关键词:  水稻  稻瘟病抗性  Pi25  分子标记辅助  中早39
DOI:10.13430/j.cnki.jpgr.20191219001
投稿时间:2019-12-19修订日期:2020-01-08
基金项目:国家重点研发计划(2017YFD0100301);浙江省农业(粮食)新品种选育重大科技专项(2016C02050-4);浙江省自然科学基金项目 (LQ17C130005);国家自然基金项目(31801681);农业部西南作物有害生物综合治理重点实验室开放基金项目(XNYH2016-1)
Improving The Blast Resistance of Super Early-season Rice Variety Zhongzao39
CAO Ni, JI Zhi-juan, ZENG Yu-xiang, ZHENG An-fu, FANG Jing-jing, YANG Chang-deng, LIANG Yan
(State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 330045)
Abstract:
Rice blast is one of the most devastating diseases in rice production. Although the commercially-released resistant cultivars often become susceptible in 3-5 years due to the rapid evolution of M. oryzae isolates, breeding for resistant variety is still the most economical and effective approach to control blast disease. In this study, the donor line R6, which harbors rice blast resistance gene Pi25, was crossed with the super early-season rice Zhongzao39 (the receptor and recurrent parent), followed by generations of backcrossing, self-pollination and molecular marker-assisted selection. The offspring lines were tested for rice blast resistance by spray inoculation in the greenhouse, injection inoculation in the field and natural infection in the disease nursery. Remarkably, four lines (i.e. FY82, FY90, FY125 and FY137) carrying Pi25 showed the elevation of resistance on leaf blast and neck blast. In addition, investigation of the genealogical composition and other resistance loci provided a preliminary dataset in order to decipher the genetic basis of rice blast resistance.
Key words:  rice  blast resistance  Pi25  marker-assisted selection  Zhongzao39