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普通小麦根系建成相关性状的全基因组关联分析
陈贵菊1, 靳义荣2, 刘彩云2, 贾德新2, 樊庆琦3, 刘金栋4, 刘鹏2
0
(1.济宁市农业科学研究院;2.德州市农业科学研究院;3.山东省农业科学院作物研究所;4.中国农业科学院 深圳农业基因组研究所)
摘要:
根系建成(RSA, Root system architecture)决定根系系统的构成,在作物生长发育过程中起着不可替代的作用。解析小麦根系建成遗传机制、选育具有较好根系建成的品种对于小麦高产、抗逆育种具有十分重要的意义。全基因组关联分析(GWAS)是解析小麦复杂数量遗传性状遗传机制的有效方法。本研究基于GWAS 方法,发掘RSA 相关性状关联位点,以期为小麦RSA 分子育种提供参考。对160 份来自于河南和山东等地的小麦品种RSA 相关性状(总根长、总根表面积、总根体积、平均根直径和根尖数)进行统计评价,并结合660K SNP 芯片数据进行GWAS 分析。检测到23 个关联位点,分布于1A、2A、2B、3B、4A、5A、5B、5D、6A、6B 和7B 染色体上,解释7.2%-12.8%的表型变异。其中,11 个位点与已报道的位点一致,其他12 个位点为新的位点。本研究对于解析根系建成遗传机制,选育高产、抗逆小麦品种具有重要意义。
关键词:  普通小麦  根系建成  全基因组关联分析  660K SNP 芯片
DOI:10.13430/j.cnki.jpgr.20191126002
投稿时间:2019-11-26修订日期:2020-01-24
基金项目:山东省重点研发计划项目(2017GNC10107);山东省西部经济隆起带基层科技人才支持计划项目(XB2018FW029);国家重点研发计划项 目(2017YFD0100600); 山东省现代农业产业技术体系小麦创新团队建设项目(SDAIT-01-17);山东省农业科学院农业科技创新工程项目 (CXGC2016B01);山东省自然科学基金项目(ZR2017BC015);德州市农业科学院资助项目(2019-01)
Genome-wide Association Study of Root System Architecture Related Traits in Common Wheat (Triticum aestivum L.)
CHEN Gui-ju1, JIN Yi-rong2, LIU Cai-yun2, JIA De-xin2, FAN Qing-qi3, LIU Jin-dong4, LIU Peng2
(1.Jining Academy of Agricultural Sciences, Jining;2.Dezhou Academy of Agricultural Sciences, Dezhou;3.Shandong Academy of Agricultural Sciences, Jinan;4.Agricultural Genomics Institute at Shenzhen, Chinese, Academy of Agricultural Sciences (CAAS), Shenzhen)
Abstract:
The root system architecture (RSA) determines the composition of the root system and plays an irreplaceable role in crop growth and development. It is of great significance to analyze the genetic mechanism of wheat root architecture and the selection of varieties with better RSA traits in breeding for elite wheat varieties. Genome-wide association study (GWAS) is an effective method to analyze the genetic mechanism of complicated quantitative traits (i.e. RSA) in wheat. In the study, GWAS for RSA-related traits(total root length, total root surface area, total root volume, average root diameter and number of root tips) were conducted in 160 wheat accessions from Henan and Shandong, which were previously genotyped by wheat 660K SNP chip. Twenty-three loci associated with RSA related traits were detected from chromosomes 1A, 2A, 2B, 3B, 4A, 5A, 5B, 5D, 6A, 6B and 7B, and each explained the phenotypic variation variable from 7.2% to 12.8%. Among them, eleven loci are found to coincide with the formerly-reported loci, and the remaining 12 loci are newly identified. Taken together, this study is of significance to uncover the genetic structure of the root architecture with a potential in future breeding of high-yield and stress-resistant wheat cultivars.
Key words:  common wheat  root system architecture (RSA)  GWAS  660K SNP chip