Yeast Two-Hybrid Screening of the Proteins Interacting with AhMYB44 from Peanut
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1 Jiangsu Academy of Agricultural Sciences,Nanjing 210014;2 Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Wuhan 430000

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Special Fund for Modern Agroindustry Technology Research System of China (CARS-13), Independent Innovation Fund of Agricultural Science and Technology of Jiangsu Province(cx(18)2015), Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, P.R. China(KF2019011)

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    Abstract:

    We previously cloned the full-length coding sequence of the gene AhMYB44,which showed a stress-induced expression from peanut drought transcriptome sequencing datasets. In order to investigate the function of AhMYB44,a yeast two-hybrid system was deployed for identifying its interacting proteins. The results showed that the bait vector pGBKT7-AhMYB44 was not toxic and showed no self-activation activity. Sixteen coding sequences were identified by screening of cDNA libraries which were constructed from a drought and high-salt stress of peanut. We further conducted an in-silico analysis of their homologous proteins,such as plant transcriptional regulation,growth and development,signal transduction,biotic and abiotic stress responses. Out of that,the candidate gene(No.46)was annotated with the abiotic stress,and encoded for a calcium-dependent phospholipid binding protein(ANNEXIN). Therefore,we speculated that AhMYB44 maybe participate into regulation of peanut stress response process through intracellular calcium signal. This result might provide a reference for further research on the function and mechanism of AhMYB44.

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History
  • Received:October 29,2019
  • Revised:October 29,2019
  • Adopted:November 07,2019
  • Online: January 17,2020
  • Published:
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