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李媛1, 吕洪坤2, 张兴伟1, 杨爱国1, 冯全福1, 戴培刚1, 刘国祥1, 佟英1
为将“第三次全国农作物种质资源普查与收集行动”重庆项目组收集到的 63 份烟草种质资源入国家种质资源库进 行编目和保存,对其进行田间鉴定和拍照记录。形态特征和主要农艺性状的遗传多样性分析表明,供试种质的数量性状和质 量性状遗传多样性丰富,质量性状以叶形的多样性指数最高,数量性状中叶长、叶宽和茎围的多样性指数较高。聚类分析将 新收集烟草种质资源分为 4 个类群,其中第Ⅰ类群以黄花烟为主,第Ⅲ类群的植株最高、叶片最大,可用于高产育种。主要 病毒病的抗性鉴定发现,有 11 份种质 TMV 和 CMV 的抗性均较强,其中 2 份种质对 TMV 免疫且高抗 CMV,可用于抗病育 种。另外,通过田间表型和 SSR 分子标记对拟编目资源进行鉴定整理,剔除了重复的种质资源。因此,烟草种质资源的普查 收集工作对于丰富国家烟草种质资源库和新品种选育具有重大意义。
关键词:  烟草  种质资源  遗传多样性  分子鉴定
基金项目:农业农村部物种资源保护费项目(2017NWB043);科技部种质资源平台运行服务项目(NICGR2017-18);中国烟草总公司海南省公司科 技计划项目(201846000024055);中国烟草总公司四川省公司科技项目(SCYC201903)
Evaluation and Cataloguing of Newly Collected Tobacco Germplasm Resources in Chongqing, China
LI Yuan1, LV Hong-kun2, ZHANG Xing-wei1, YANG Ai-guo1, FENG Quan-fu1, DAI Pei-gang1, LIU Guo-xiang1, TONG Ying1
(1.Institute of Tobacco Research of CAAS;2.Hainan Cigar research Institute Hainan Provincial Branch of China National Tobacco Corporation)
In order to catalog and preserve 63 tobacco germplasm accessions, which were collected by Chongqing Project Group in a frame of the Third National Survey and Collection Action on Crop Germplasm Resources, we conducted the field experiments for identifying the phenotypic and marker-assisted genetic diversity. Morphological characteristics and main agronomic traits were surveyed and analyzed. These tested germplasm accessions represented abundant genetic diversity at both quantitative traits and qualitative traits. Specially for qualitative traits, leaf shape had the highest diversity index, followed by Leaf length, leaf width and stem girth. Clustering analysis suggested four groups of these newly-collected tobacco germplasm accessions. The germplasms resided to the first group were mainly rustica, while the germplasms from the third group showed higher plant height and bigger leaves with a potential for high-yield breeding. Tests for disease resistance indicated that 11 accessions were resistant to TMV and CMV. Particularly, two of them were free of TMV infection and highly resistant to CMV, which could be used for disease resistance breeding. By taking use of field phenotypic datasets and genotyping results using SSR markers, the germplasm accessions showing identical were excluded for cataloglization and conservation into the National Bank of Tobacco Germplasm Resources. Therefore, survey and collection of tobacco germplasm resources is of great significance, in order to enrich the genetic diversity of Tobacco Germplasm Resources and benefit for tobacco breeding.
Key words:  tobacco  germplasm resources  genetic diversity  molecular identification