Stress-related Expression Profile of F-box/Kelch Gene TaFKOR23 in Wheat and Molecular Characterization of the Interacting Target Protein
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1.College of Life Sciences, Hebei Agricultural University / Hebei Key Laboratory of Plant Physiology and Molecular Pathology, Baoding 071001;2.College of Plant Protection, Northwest A&F University / State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100;3.Research Center for Engineering and Technology of Biological Control of Pests and Diseases of Agricultural Crops of Hebei Province, Baoding 071001

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    Abstract:

    F-box protein is responsible for the recognition of substrates to be degraded in the process of ubiquitin-mediated protein degradation. In recent years, the role of F-box protein in plant resistance to stresses has attracted more and more attention. In order to understand the expression profile and molecular mechanism of F-box members in response to biotic/abiotic stresses in wheat, TcLr15 - a near isogenic line against leaf rust pathogen, was used as the material to obtain the complete coding region (encoding a polypeptide of 421 aa) of wheat F-box gene TaFKOR23. Wheat TaFKOR23 includes 1 F-box domain at N-terminal and 2 Kelch domains at C-terminal, which means that the wheat TaFKOR23 belongs to F-box/Kelch subfamily. Phylogenetic tree showed that wheat TaFKOR23 had extremely high homology with F-box/Kelch-repeat protein OR23 from Aegilops tauschii, and had high homology with that from Brachypodium distachyon. Expression profiles of the gene in TcLr15 plants after treatment with leaf rust pathogen, 3 hormones (ABA, SA and MeJA) and abiotic stresses (NaCl and PEG) were analyzed by qRT-PCR in the present study. No significant difference was observed for TaFKOR23 transcripts accumulation between compatible and incompatible combinations; TaFKOR23 showed up-regulated expression after treatment with the 3 hormones and NaCl, but less affected by PEG; TaFKOR23 prominently expressed in flag leaves than in other parts of wheat. Target proteins interacting with TaFKOR23 were screened and identified by yeast two-hybrid, 11 kinds of target proteins were obtained by library screening, and further results showed that TaFKOR23 can interact with TaSKP1, TaSLY and TaChitinase. The research results laid a solid foundation for further understanding the function and metabolic network of F-box/Kelch genes in wheat, and broadened the functional knowledge of Kelch like F-box genes in plant.

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History
  • Received:June 26,2019
  • Revised:December 07,2019
  • Adopted:December 12,2019
  • Online: May 18,2020
  • Published:
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