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青藏高原8种野生葱属植物挥发性成分研究
关志华, 王忠红, 朗杰, 德庆措姆
0
(西藏农牧学院植物科学学院,林芝 860000)
摘要:
为促进青藏高原野生葱属植物资源的开发利用,采用动态顶空-气相色谱/质谱联用仪法测定了 8 种产于西藏的野生 葱属植物的挥发性成分,并通过 NIST 11 和 Wiley 7n 标准谱库对其组分进行了定性分析,及用色谱峰面积归一化法对各成分 进行了相对定量分析。结果表明,在杯花韭、大花韭、野黄韭、野葱、太白韭、天蓝韭、粗根韭、多星韭的叶中共检测到 25 种挥发性成分,其中,野黄韭所含的挥发性成分种类最多(14 种),太白韭的最少(3 种),其他种的在 10 种左右;乙醚和二 甲基二硫化物在 8 种野生葱属植物中均有检出,但相对含量差异大;丙硫醇仅在杯花韭和野黄韭中有检出,丙醛、甲基硫杂 丙环、二丙基三硫化物、2,4-二氢-4-甲基-3H-1,2,4-三唑-2-硫酮、3-氨基-2-硫代-4-噻唑啉酮、1-氯-1-丙烯等仅在野黄韭中有检 出,甲硫基乙睛和二烯丙基硫醚则仅在大花韭中有检出,甲基丙基硫醚仅在杯花韭中有检出,己醛仅在粗根韭的肉质根中有 检出,正辛烷和 2-氟丙烯仅在野葱茎中有检出。在同一种野生葱属植物的不同部位中,所含的挥发性成分为:野黄韭地下茎 和叶中分别含有 2 种和 14 种;野葱地下茎和叶中分别含有 15 种和 12 种;太白韭地下茎和叶中分别含有 6 种和 3 种;粗根韭 茎(地上和地下均有)、肉质根、叶分别含 10、9、8 种。
关键词:  青藏高原  野生葱属植物  挥发性成分
DOI:10.13430/j.cnki.jpgr.20190609002
投稿时间:2019-06-09修订日期:2020-04-24
基金项目:西藏野生葱属植物种质资源分类、描述符建立及综合评价(31260478)
Study on the Volatile Components of 8 Wild Allium Plants from Qinghai-Tibet Plateau
GUAN Zhi-hua, WANG Zhong-hong, LANG jie, DEQING Cuomu
(Plant Sciences College, Tibet Agriculture & Animal Husbandry University, Nyingchi 860000)
Abstract:
In order to promote the development and utilization of wild Allium resources in the Tibetan Plateau, the volatile components of eight wild Allium species in Tibet were measured by dynamic headspace gas chromatography / mass spectrometry. The components identified were determined by NIST 11 and Wiley 7n standard library, and the quantities were calculated by peak area normalization. Twenty-five volatile compounds were detected in the leaves of Allium cyathophorum, A. macranthum, A. rude, A. chrysanthum, A. prattii, A. cyaneum, A. fasciculatum and A. wallichii. Fourteen volatile components were detected in A. rude, three in A. prattii, approximately ten in other species. The diethyl ether and dimethyl disulphides were found in eight wild Allium species with variable levels on content. The propyl mercaptan was specifically detected in A. cyathophorum and A. rude, and the compounds including propanal, methylsulfur heterocyclic ring, dipropyl trithiolides, 2,4-dihydro-4-methyl-3H-1,2,4-triazole-2-thionone, 3-amino-2-thiazolinone, and 1-chloro-1-propylene were extensively detected in A. rude. The trimethylamine and diallyl thioether were detected in A. macranthum, methylpropyl thioether in A. cyathophorum, hexaldehyde in roots of A. fasciculatum, as well as positive octane and 2-fluoropropene in stems of A. chrysanthum. In A. rude, two and fourteen volatile components were detected in underground stems and leaves, respectively. 15 and 12 volatile components in underground stems and leaves of A. chrysanthum were identified. The volatile substances in different parts of the same wild Allium are as follows: there are 2 kinds and 14 kinds in the underground stems and leaves of A. rude; 15 kinds and 12 kinds in the underground stems and leaves of A. chrysanthum; 6 kinds and 3 kinds in the underground stems and leaves of A. prattii; as well as there are 10, 9 and 8 kinds in the Stem (both above and below ground) , fleshy roots and leaves of A. fasciculatum respectively.
Key words:  Qinghai-Tibet Plateau  wild Allium plants  the volatile components