Application of resistant varieties is one of the most economical and effective measure to control rice bacterial leaf streak (BLS). Screening and utilization of resistant germplasm is the fundamental work for developing resistance varieties. 59 of domestic rice varieties or hybrid combinations,318 of foreign germplasms and 13 of near-isogenic lines (NILs) with resistance to bacterial blight (BB) were evaluated on their resistance of BLS. Among the tested rice entries, all of domestic varieties showed susceptible to BLS. 82 of rice germplasms were highly resistant or resistant to BLS, including Popong、IR63372-8、Weda Heenati、Balayan、Gharibe etc. Only BB near-isogenic line IRBB5 was resistant to BLS, while IRBB21 and IRBB23 with broad spectrum resistance to bacterial blight were highly susceptible to BLS. 95 of rice germplasms were selected to analyze the association between the resistances of BB and BLS. Association analysis of 82 rice germplassms and 13 of BB NILs both showed no correlation between two diseases, with the index r =0.103 (P value 0.358>0.05) and index r=0.527 (P value 0.064>0.05) respectively. 79 of rice germplasms with the resistance to BLS were detected with functional molecular markers of xa5 the result indicates that 30 of the tested germplasms habour the xa5 gene, while the other 49 of tested entries do not have the tested gene, which could be the important resoures for the genes mining and the rice breeding with the resistance against BLS.