As the ancestor of cultivated soybean, annual wild soybean (Glycine soja Sieb et Zucc) has rich genetic diversity, high protein content, strong tolerance to stresses, etc. In northeast China, the wild soybean distributed widely, but the studies on trait identification and breeding application of the wild soybean germplasm are inadequate. In the current study, 30 accessions of wild soybeans which were collected from upper and middleSreachesSofSthe Heilongjiang River and had similarSgrowth duration. Drought tolerance of these accessions were evaluated both in normal irrigation and drought stress treatments using multiple indices. The results showed that there were differences in responses of various traits of wild soybeans to drought stress, i.e., yield and plant height were sensitive to drought stress whereas 100-seed weight and maturity traits were insensitive. The tested wild soybean germplasm were classified into five groups according to the drought tolerance comprehensive value (D). Among the 30 accessions of the wild soybeans, 2 accessions (Jin 05015 and C05038 C) were classified into grade I (highly-tolerant), 4 grade II (medium-tolerant), 12 grade III (weakly-tolerant), 10 grade IV (water-sensitive) and 2 grade V (highly-sensitive). D value, the comprehensive index of drought tolerance, is positively correlated with vegetative growth period (V), branch number and seed yield per plant, and is negatively correlated with reproductive period (R) and the ration of R to V (R/V), indicating that wild soybeans with longer vegetative growth period, shorter reproductive period and smaller R/V ratio are more tolerant to drought. It was proposed that the D value as the comprehensive index based on multiple traits is a reliable indicator for evaluating the drought tolerance of wild soybeans. The duration of vegetative growth, R/V ration, branch number and seed yield per plant can be used as simple indices for drought tolerance evaluation.