Sequence-related amplified polymorphism (SRAP) analysis was carried out to evaluate the genetic diversity and genetic relationships among 33 Anthurium andraeanum cultivars. Out of 100 pairs of primers screened twenty-six pairs of SRAP primers were used to analyze genetic diversity of the tested materials. The results showed that: (1) Twenty-six primer pairs amplified out 366 bands, out of which 314 bands were polymorphic bands, the percentage of polymorphic bands was 85.79%. The number of bands ranged from 9 to 23 with an average of 14.1 bands and 12.1 polymorphic bands per primer. (2) According to the SRAP amplification results, the genetic similarity coefficient among tested cultivars was in a range from 0.55 to 0.94, a dendrogram produced using unweighted pair group method using arithmetic averages (UPGMA),and 33 Anthurium andreanum cultivars were divided into five groups when genetic similarity coefficient was given as 0.786. The Anthurium cultivars showed a high genetic diversity, and the results would facilitate variety identification and cross breeding.