456 S.spontaneum L. derived from various geographical origins were evaluated by mutation analysis and cluster analysis based on 13 phenotype traits, to create initial knowledge with better understanding of their genetic diversity, and to provide useful informations for variety improvement in breeding programs. The results showed that the very high diversity was detected on all of the traits of 456 S.spontaneum L. in height of plant, length of leaf, width of leaf, brix , between 0.97 to 7.92 in Shannon index. It has a widely range of variation in mosaic disease, eyespot disease, tumblebug and so on. There is a large genetic variation among S.spontaneum L. resources in geographic origin, and it is the morst abundance diversity of the S.spontaneum L.groups in Yunnan.By cluster analysis,the results showed that the S.spontaneum L. resources may be divided into 3 categories, Yunnan for a major category and the S.spontaneum L. introduced from abroad for the second category, others for the third category.Which all have the highly significant differences in plant growing characteristic and brix.