To get potential gene resources from wild germplasm for broadening sugarcane genetic base, crosses between sugarcane (Saccharum spp.) and intergeneric hybrid complex (Erianthus arundinacius×Saccharum spontaneum) were carried out and their progenies were then identified by microsatellites (SSR) and sequence-related amplified polymorphism (SRAP) molecular markers in this study. 34 true hybrids were identified and the results confirmed that utilizing both S. spontaneum and E. arundinaceus in introgression breeding programs to improve sugarcane are viable.