Seed storage proteins of 260 maize inbred lines were analyzed by acid polyacrylamide gel electrophoresis(A-PAGE) in order to clarify genetic diversity of these lines.Globulin and glutelin showed high polymorphism in electrophoretic pattern in these lines,and both were quite similar.The number of globulin bands,95% of which were polymorphic in these lines,was more than that of glutelin bands.Clustering results showed that the clusters were not corresponding to heterotic groups and pedigrees,which suggested that the A-PAGE of seed storage proteins might not be a good method for large-scale analysis of genetic diversity and heterotic grouping in maize germplasm.