11 corn inbred lines with different Ht gene background were used for detecting the practicability of 5 molecular markers linked to Ht2 and Ht3 genes.Primers,umc1202,bnlg1152,umc1149,SD-06633 and bnlg1666 were detected for their specific to Ht gene.No specific amplification was found in all of the primers.Analysis of the fragments amplified by primer SD-06633,linked to Ht2 gene,showed that the sequence identity was 98.73% between lines with Ht2 and HtN gene.It means that the primer,SD-06633,can not amplified specially for Ht2 gene.None of the 5 primers can be used to identify the present of Ht2 or Ht3 gene in corn.