Genetic population of F2 and its corresponding F2:3 lines derived from a hybridization combination of Lumianyan22 and Luyuan 343 were used to evaluate the fiber quality and detect the related introgression genes by SSR markers.10 SSR loci were proved to be derived from the Island cotton primogenitor by compared all polymorphic markers.10 SSR loci were proved to be derived from the Island cotton primogenitor by compared all polymorphic marker loci with the Island cotton primogenitor(Ashimouni,a famous Egypt Island cotton cultivar) and the standard genetic line(TM-1) of Upland cotton.There were at least 6 marker loci,which involved in 4 chromosomes,significantly related to different parameters of high fiber quality in this introgression upland cotton germplasm.Statistical analysis and linkage analysis based on the same population showed that,marker loci BNL2986 which related to fiber elongation(R2=5.87%) and NAU751 which related to fiber length(R2=6.62%) and fiber fineness(R2=6.01%) were linked with 17.7cM of genetic distance and located on Chromosome 16.BNL3590 which related to fiber maturation(R2=8.62%) and BNL3971 which related to fiber maturation(R2=15.0%) and fiber elongation(R2=9.79 %) were linked with 4.5 cM and located on Chromosome 2.While BNL3279 which related to fiber strength(R2=8.12%) and BNL827 that related to fiber fineness(R2=13.94%) were dispersed in LGD02 and chromosome 25 respectively.